H-2Db Mouse gp100 (EGSRNQDWL) MHC Tetramer
CAT#: MHC-LC211
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of Mouse gp100-derived peptide of EGSRNQDWL sequence covering 25-33 and H-2Db molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to endoplasmic reticulum & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/1898_C6_19_cr5ba8a6f6e250d_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_A_7_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_A_4_6.jpg
Normal Tissue
Figure 4 Skin
Melanocytes
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185664-PMEL
❮
❯
Specifications
- Allele
- H-2Db
- Class
- Class I
- MHC Species
- Mouse
- Antigen
- Mouse gp100
- Antigen Species
- Mouse
- Peptide
- EGSRNQDWL
- Range
- 25-33
- Conjugate
- APC
- Application
- FCM
Target
- Antigen Introduction
- This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
- Alternative Names
- Pmel; premelanosome protein; Si; Silv; gp87; gp100; Pmel17; D12S53Eh; D10H12S53E
- Gene ID
- 6490
- UniProt ID
- P40967
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Customer Review
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Suitable to detect gp100-specific T cells
The H-2Db Mouse gp100 MHC Tetramer has been invaluable in my research. It has significantly improved our ability to detect gp100-specific T cells with high sensitivity in our melanoma studies.
Reliable in FC
I liked how well the tetramer performed in flow cytometry assays. Clear and distinct population separation allowed us to better quantify antigen-specific T cells.
Excellent for FC
Perfect for flow cytometry! This tetramer has streamlined our validation process for gp100-targeted immunotherapies. Highly recommend to fellow researchers.
Q&As
-
Is it possible to apply the H-2Db Mouse gp100 MHC Tetramer for flow cytometry purposes?
A: Yes, it is optimized for flow cytometry to analyze the presence and frequency of gp100-specific CD8+ T cells in a given sample.
-
With a single vial of H-2Db Mouse gp100 MHC Tetramer, how many tests can be run?
A: A standard vial typically allows for 50 tests, depending on the recommended dilution and cell sample size.
-
Is it feasible to image the distribution of T cells in vivo using this tetramer?
A: In vivo imaging would require specialized modifications and conjugation to imaging agents; typically, it is used for ex vivo analyses.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
MSDS
COA
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| HPAB-0046-LSX-F(E) | Mouse Anti-PMEL Recombinant Antibody; Fab Fragment (HPAB-0046-LSX-F(E)) | ELISA, WB, FC | Mouse Fab |
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