H-2Kd/Enhanced Green Fluorescent Protein (HYLSTQSAL) MHC Tetramer
CAT#: MHC-LC689
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of EGFP-derived peptide of HYLSTQSAL sequence covering 200-208 and H-2Kd molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specifications
- Allele
- H-2Kd
- Class
- Class I
- MHC Species
- Mouse
- Antigen
- EGFP(Enhanced Green Fluorescent Protein)
- Peptide
- HYLSTQSAL
- Range
- 200-208
- Conjugate
- PE
- Application
- FCM
Target
- Antigen Introduction
- The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.
- Alternative Names
- EGFP;Enhanced Green Fluorescent Protein; GFP
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Essential for EGFP Studies
This MHC tetramer has been indispensable in our research involving EGFP-expressing cells. Its ability to specifically detect EGFP-specific CD8+ T cells has greatly enhanced our understanding of immune responses in our gene therapy models. The tetramer's high sensitivity and specificity have provided us with reliable data that's critical for our studies.
Excellent Technical Support
Creative Biolabs' technical support team was incredibly helpful when we needed assistance optimizing our staining protocol. Their quick and detailed responses ensured we achieved the best possible results. The customer service has been outstanding and reflects the quality of the product.
Q&As
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How can this tetramer be utilized in immunological research applications?
A: This tetramer is ideal for flow cytometry analysis to identify and quantify EGFP-specific CD8+ T cells in mice. It aids in studying T cell responses in models where EGFP is used as a reporter gene, facilitating research on immune responses to gene therapy vectors, transgenic models, and cellular tracking.
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Is this tetramer compatible with standard flow cytometry protocols?
A: Yes, the H-2Kd EGFP (200-208) MHC Tetramer is designed to integrate into standard flow cytometry protocols. It is available conjugated to PE, enabling multicolor staining and simultaneous analysis of multiple cellular markers alongside antigen-specific T cell detection.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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| DrMAB-1007 | Human Anti-EGFP Recombinant Antibody (clone G3) | ELISA, ICC, IP, WB | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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