CBGlyco™ Hydrazide-Biotin (CAT#: Glyco-025CL)

Hydrazide-Biotin is the shortest and simplest hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.
This biotin hydrazide reagent enables simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies because biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.


Specific Inquiry
  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 100 mg
  • Label or Dye
  • Biotin
  • Spacer
  • Short
  • Solubility
  • DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
  • Cell Permeability
  • Cell-Permeant
  • Chemical Reactivity
  • Carboxylic Acid, Ketone, Aldehyde
  • Contents & storage
  • Store at 4°C.

Background

  • Description
  • This biotin hydrazide reagent enables simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies because biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.
  • Properties

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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