CBGlyco™ IP Kit (CAT#: Glyco-030CL)

IP Kit provides components for effective immunoprecipitation (IP and co-IP) based on small-scale, covalent, affinity-resin immobilization of antibodies and other glycoproteins via oxidized sugar groups.

Specific Inquiry
  • Datasheet
  • MSDS
  • COA


  • Product Size
  • 25 reactions
  • Technique
  • Immunoprecipitation (IP)
  • Assay Type
  • Affinity Purification
  • Target Specificity
  • Not Target-Specific
  • For Use With (Equipment)
  • Microcentrifuge
  • Contents & storage
  • Sufficient For: 25 IP/Co-IP reactions, each using 20 µL antibody-coupled resin
    • UltraLink Hydrazide Resin, 0.5 mL
    • Coupling Buffer, 50 mL
    • Aniline, 0.2 mL
    • Sodium meta-periodate, 0.5 g
    • Wash Buffer, 60 mL
    • Zeba Spin Desalting Columns,0.5 mL, 25 columns
    • IP Lysis/Wash Buffer, 50 mL
    • PBS Pack (makes 500 mL), 1 pack
    • Elution Buffer, pH 2.8, 50 mL
    • Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL
    • Spin Columns-Screw Caps, 25 columns
    • Collection Tubes, 2 mL, 100 tubes

    Store at 4°C.


  • Description
  • The Kit uses hydrazide-activated UltraLink Resin to immobilize and perform 25 IP or Co-IP assays with different antibodies or glycoprotein ligands. The method is ideal for polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Monoclonal antibodies that contain adequate carbohydrates are also effective. Antibodies immobilized by this hydrazide-to-carbohydrate method have unobstructed antigen-binding sites and optimal purification capability. The prepared glycoprotein or antibody columns can be regenerated and reused at least five times for immunoprecipitation, co-immunoprecipitation or pull-down affinity-purification without significant loss in binding capacity.
    Kit contains resin, buffers and reagents for immobilization; desalting columns; IP lysis/wash and elution buffers for immunoprecipitation; microcentrifuge spin columns and collection tubes; and loading buffer for SDS-PAGE
    The IP Kit method results in covalent attachment of oxidized sugar groups of an IP antibody (or any other glycoprotein with oxidizable carbohydrate groups) to the hydrazide resin. The antibody resin is then incubated with the sample that contains the protein antigen of interest, allowing the antibody-antigen complex to form. After washing to remove non-bound components of the sample, the antigen is recovered by dissociation from the antibody with elution buffer supplied in the kit. The entire procedure is performed in a microcentrifuge spin cup, allowing solutions to be fully separated from the resin with a brief centrifugation. Only the antigen is eluted by the procedure, enabling it to be identified and further analyzed without interference from antibody fragments.


Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for ""

Customer Reviews and Q&As

Submit a review or a question
Thare are currently no Customer reviews or questions for Glyco-030CL. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
REImmunohistology - Resin Sections
IRMAImmunoradiometric Assay
SHIn situ hybridization
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
RIRadial Immunodiffusion
SPRSurface Plasmon Resonance
WBWestern Blot

Send Inquiry

© 2022 Creative BioLabs. All Rights Reserved
  • 0
  • 0