Jacalin Agarose (CAT#: Glyco-061CL)

Jacalin Agarose provides for specific affinity purification of human IgA from serum or colostrum or efficient removal of contaminating IgA from IgG samples.

  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 5 mL
  • Format
  • Bottle(s)
  • Column Type
  • Affinity, Agarose Resin
  • Immobilized Phase
  • Lectin
  • Contents & storage
  • Upon receipt store product at 4°C. Product is shipped at ambient temperature. Do not freeze product.

Background

  • Description
  • Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilized on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

    Binding of IgA toimmobilized jacalin occurs at physiologic pH and ionic strength, as inphosphate-buffered saline (PBS). Elution of bound IgA occurs withcompetitor ligand (e.g., 0.1M melibiose or 0.1 M alpha-D-galactose) inPBS. IgD is reported to bind to jacalin (Aucouturier, et al., 1987).

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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