CBGlyco™ Krypton Fluorescent Protein Stain (CAT#: Glyco-046CL)
Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.
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Specifications
- Product Size
- 20 mL
100 mL
500 mL
- Target Molecule
- Protein
- Label or Dye
- Proprietary Mix
- Detection Method
- Fluorescent
- Detection Location
- In-Gel Detection
- Contents & storage
- Upon receipt store at 4°C.
Background
- Description
- This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.
For best results, detect bands using visible laser-based imagers equipped with a 532nm laser light source. Although 580nm-filters are optimal for emission, 600nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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