CBGlyco™ Lumio Green Detection Kit (CAT#: Glyco-054CL)

The Lumio Green Detection Kit is a sensitive and highly specific kit for labeling Lumio fusion proteins prior to electrophoresis. The Lumio Green Kit enables immediate visualization of Lumio fusion protein bands directly in a polyacrylamide gel.

  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 1 kit
  • Target Molecule
  • Proteins (Lumio-Tagged) , Proteins (TC-Tagged)
  • Label or Dye
  • Lumio Green
  • Detection Method
  • Fluorescent
  • Detection Location
  • In-Gel Detection
  • Contents & storage
  • The Lumio Green Detection Kit contains one vial of Lumio Green Detection Reagent, five vials of Lumio Gel Sample Buffer, and one vial of Lumio In-Gel Detection Enhancer.
    Sufficient reagents are included in the kit for 100 in-gel detections or 20 in vitro transcription-translation labeling reactions.
    Store at -20°C. All components are guaranteed stable for 6 months when properly stored.

Background

  • Description
  • • Label proteins fused to a Lumio sequence
    • Visualize results immediately after electrophoresis—eliminate gel staining and western blotting
    • Detect nanogram levels of protein with a UV transilluminator, equipped with a standard camera, or a laser-based scanner
    The Lumio Green Detection Reagent, included in the Lumio Green Detection Kit, can also be used for real-time detection of protein synthesis during in vitro transcription-translation reactions.
    To detect using the Lumio Green Detection Kit simply:
    1. Add the Lumio Green Detection Reagent and the optimized Lumio Sample Buffer to your protein sample and heat for 10 minutes at 70°C.
    2. Add the Lumio In-Gel Detection Enhancer to your sample mix and incubate for 5 minutes at room temperature.
    3. Load and electrophorese your protein gel.
    4. Visualize your Lumio fusion protein bands with a UV transilluminator, equipped with a standard camera, or laser-based scanner.
    5. After documenting your Lumio Green signal, total protein staining can be performed.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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