PE-H-2Db/Mycobacterium tuberculosis Mtb32a (GAPINSATAM) MHC Tetramer
CAT#: MHC-LC627
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of M. tuberculosis-derived peptide of GAPINSATAM sequence covering 309-318 and H-2Db molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specifications
- Allele
- H-2Db
- Class
- Class I
- MHC Species
- Mouse
- Antigen
- Mycobacterium tuberculosis Mtb32a
- Antigen Species
- Mycobacterium tuberculosis
- Peptide
- GAPINSATAM
- Range
- 309-318
- Conjugate
- PE
- Application
- FCM
Target
- Antigen Introduction
- Mycobacterium bovis (M. bovis) is a slow-growing (16- to 20-hour generation time) aerobic bacterium and the causative agent of tuberculosis in cattle (known as bovine TB). It is related to Mycobacterium tuberculosis—the bacterium which causes tuberculosis in humans—M. bovis, and can jump the species barrier and cause tuberculosis in humans and other mammals.
- Alternative Names
- M. bovis Mtb32a; Mycobacterium bovis Mtb32a; pepA; serine protease PepA
- Gene ID
- 886924
- UniProt ID
- O07175
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Good Signal for TB Antigen
I used this PE-labeled H-2Db tetramer to study the immune response to Mycobacterium tuberculosis Mtb32a. It successfully identified mouse CD8 T cells specific to the GAPINSATAM peptide, giving us clear and interpretable flow cytometry plots.
Consistent Tetramer Reagent
The product quality has been consistent. It helped us analyze individual antigen-specific T cells targeting the 309-318 region of the Mtb32a protein. The fluorescence was stable, which is critical for our comparative studies in mouse models.
Q&As
-
What specific peptide is loaded onto this H-2Db tetramer?
A: This tetramer is loaded with the peptide sequence GAPINSATAM. This sequence is derived from the Mycobacterium tuberculosis Mtb32a protein, specifically covering amino acids 309-318, and is presented by the H-2Db molecule.
-
What species is this tetramer designed to work with?
A: This product is designed for use with mouse samples. It consists of the mouse MHC Class I molecule H-2Db and is intended to recognize specific mouse CD8 T cells that react to the M. bovis/M. tuberculosis antigen.
View the frequently asked questions answered by Creative Biolabs Support.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC628 | APC-H-2Db/Mycobacterium tuberculosis Mtb32a (GAPINSATAM) MHC Tetramer | FCM |
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