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  • PE-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer

PE-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer (CAT#: MHC-LC1325)

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of H2AFX-derived peptide of RIIPRHLQL covering 78-86 and HLA-G molecule. The pMHC tetramer recognizes CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/769_B5_3_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells,non-germinal center cells and squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/166885_A_5_8_selected.jpg
Figure 4 Lymph node
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_9_8.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_3.jpg
Figure 7 Liver
Figure 7 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_5.jpg
Figure 9 Testis
Figure 9 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_6_6.jpg
Figure 10 Skin
Figure 10 Skin
(Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_1.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188486-H2AFX

Specifications

  • Allele
  • HLA-G
  • Class
  • Class I
  • MHC Species
  • Human
  • Antigen
  • H2AFX
  • Antigen Species
  • Human
  • Peptide
  • RIIPRHLQL
  • Range
  • 78-86
  • Conjugate
  • PE
  • Application
  • FCM

Target

  • Antigen Introduction
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
  • Alternative Names
  • H2AFX; H2A histone family member X; H2AX; H2A.X; H2A/X

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CAT Product Name Application Type
MHC-LC1326 APC-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer FCM
MHC-LC1327 BV421-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer FCM
MHC-LC2076 PE-G*01:01/Human H2AFX (RIIPRHLQL) MHC Tetramer FCM

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High Specificity and Affinity
Its high specificity and strong affinity for H2AFX make it a trustworthy choice for researchers looking to deepen their understanding of immune cell profiling in oncology.

Improves Experimental Precision
I have utilized various tetramers, and this one stands out for its precision and ease of integration into our existing protocols. Its impact on enhancing the clarity of data from flow cytometry analyses cannot be overstated, significantly aiding our cancer research.

Enhanced Research Outcomes
Using this tetramer, we witnessed improved outcomes in our projects, especially those related to studying DNA repair mechanisms. Its precision allows us to precisely track T cell populations with high fidelity.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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