CBGlyco™ Protein Stain (sensitive, R-250 coomassie stain) (CAT#: Glyco-041CL)

Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels.


Specific Inquiry
  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 1 L
    3 x 1 L
  • Target Molecule
  • Protein
  • Label or Dye
  • Coomassie
  • Detection Method
  • Colorimetric
  • Detection Location
  • In-Gel Detection, In-Blot Detection
  • Contents & storage
  • Upon receipt store product at room temperature.

Background

  • Description
  • Protein Stain is a coomassie dye reagent for detection of protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gels. The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The Protein Stain protocol uses simple water-wash steps rather than methanol/acetic acid fixation and destaining, which saves valuable preparation time and minimizes reagent costs.

    Protein stain is a unique formulation of Coomassie Brilliant Blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercially available coomassie-based stains. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements. The use of Protein Stain does not result in the problems (e.g., staining consistency) that can be associated with other coomassie G-250 stain preparations. In addition to faster protein band development and improved sensitivity over standard coomassie G-250 stains, Protein Stain does not require methanol/acetic acid fixation and destaining, saving valuable preparation time and minimizing reagent cost.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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