Recombinant Anti-Human GCC2 Antibody scFv Fragment
CAT#: MOB-3017z-S(P)
Recombinant Mouse Antibody scFv Fragment molecule has affinity for the Human GCC2, expressed in E. coli.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35849/381_D10_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35850/1509_D4_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35849/74610_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35849/74610_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35849/74610_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35849/74610_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35849/74610_A_6_6.jpg
Normal Tissue
Figure 8 Prostate
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35849/74610_A_3_5.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35849/74610_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135968-GCC2
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Specifications
- Immunogen
- Human GRIP And Coiled-Coil Domain Containing 2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- scFv
- Specificity
- Tested positive against native Human GRIP And Coiled-Coil Domain Containing 2
- Species Reactivity
- Human
- Applications
- Antibody assay: Flow Cytometry; Western blot; Functional Study
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- Store at -20°C. Open under aseptic conditions.
Target
- Alternative Names
- GCC2; Golgi Coiled-Coil Protein GCC185; GRIP And Coiled-Coil Domain-Containing 2; GRIP And Coiled-Coil Domain Containing 2; REN53; GCC185; Ran-Binding Protein 2-Like 4; KIAA0336; CTCL Tumor Antigen Se1-1; 185 KDa Golgi Coiled-Coil Protein; GCC Protein, 18
- Gene ID
- 9648
- UniProt ID
- Q8IWJ2
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Remarkable Golgi Research Tool
The GCC2 Antibody scFv Fragment has proven indispensable in our research. Its high specificity to GCC2 has enabled us to map and analyze the organization and function of the Golgi apparatus with unprecedented clarity, driving our understanding of intracellular trafficking forward.
Efficient and Reliable
Using this scFv fragment for Western blotting has significantly enhanced our protein analysis capabilities. Its high purity ensures clear results, and the reliable production consistency removes any performance variability we used to encounter with other antibodies.
Perfect for Flow Cytometry
We've successfully incorporated this scFv fragment into our flow cytometry experiments. Its small size and specific binding have allowed us to achieve greater detail in cellular population studies, particularly those requiring precision in cell compartment targeting.
Q&As
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Which applications is this scFv fragment used for?
A: This scFv fragment is frequently utilized in antibody assays such as flow cytometry, Western blotting, and functional studies. Its specificity facilitates precise research into cellular localization and protein interactions within the Golgi apparatus.
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What are the scFv fragment's production and purity characteristics?
A: The antibody is expressed in E. coli, ensuring high production efficiency. Its purity exceeds 95%, as confirmed by SDS-PAGE with Coomassie Brilliant Blue staining, providing reliable results in various assays.
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What makes GCC2 a significant protein for study?
A: GCC2 plays a pivotal role in Golgi apparatus functioning and protein trafficking, making it an important marker for studies involving cell organization and secretory pathways, into which this scFv fragment provides insights.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "GCC2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3017z | Recombinant Anti-Human GCC2 Antibody | ELISA, WB, IF, FuncS | IgG |
| CBPAB-055LC | Rabbit Anti-GCC2 Polyclonal Antibody | IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3017z-F(E) | Recombinant Anti-Human GCC2 Antibody Fab Fragment | IP, FuncS | Fab |
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