Mouse Anti-NNMT Recombinant Antibody (clone 27D6)
CAT#: MOB-3452z
This product is a mouse antibody that recognizes human NNMT. It can be used in the following applications: WB, ELISA, FCM, ICC, IF.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59180/1184_C12_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59180/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59180/132915_B_8_5.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59180/132915_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59180/132915_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HSkMC, LHCN-M2, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166741-NNMT
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Specifications
- Immunogen
- Human Nicotinamide N-Methyltransferase
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a, κ
- Antibody Isotype
- IgG2a, κ
- Specificity
- Human NNMT
- Species Reactivity
- Human
- Clone
- 27D6
- Applications
- WB, ELISA, FC, ICC, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry Assay, Immunofluorescence Assay.
Target
- Alternative Names
- NNMT; EC 2.1.1.1; Nicotinamide N-Methyltransferase
- UniProt ID
- P40261
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Versatile Antibody For Metabolism
Our lab investigates metabolic enzymes, and this antibody was excellent for detecting Nicotinamide N-Methyltransferase. We successfully applied it in both Western Blot and ELISA formats. The band specificity in the blot was high, aligning perfectly with the predicted molecular weight of the target protein.
Great Immunofluorescence Staining
We used the 27D6 clone for immunofluorescence assays to localize NNMT within liver tissue sections. The staining pattern was sharp and consistent. It is reassuring to work with a recombinant antibody that minimizes lot-to-lot variation, ensuring our imaging data remains reproducible over time.
Q&As
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Is this antibody suitable for detecting NNMT in Western Blots?
A: Yes, this recombinant mouse antibody (clone 27D6) can be used for Western Blot (WB) analysis. It is also applicable for Enzyme-linked Immunosorbent Assay (ELISA), Flow Cytometry (FCM), Immunocytochemistry (ICC), and Immunofluorescence (IF) assays to detect human NNMT.
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What is the immunogen used to generate this antibody?
A: The antibody was generated using a recombinant protein corresponding to amino acids 1-264 of Human NNMT. This gene encodes the protein responsible for N-methylation enzymatic activity, using S-adenosyl methionine as the methyl donor in liver metabolism.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "NNMT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0789CT | Recombinant Mouse anti-Human NNMT Monoclonal antibody (EML734) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0396MZ | Chicken Anti-NNMT Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4883 | Anti-NNMT Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4884 | Anti-Mouse NNMT Immunohistochemistry Kit | IHC |
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