Mouse Anti-VIPR1 Recombinant Antibody (clone 18B11)
CAT#: MOB-1197z
This product is a mouse antibody that recognizes human VIPR1. It can be used in the following applications: WB, IP, IF, IHC, ELISA.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26777/60860_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Peripheral nerve/ganglion
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26777/60860_A_8_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26777/60860_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26777/60860_A_6_6.jpg
Normal Tissue
Figure 5 Skeletal muscle
Myocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26777/60860_B_3_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26777/60860_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, OE19, RT4, SK-BR-3, WM-115)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, OE19, RT4, SK-BR-3, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000114812-VIPR1
❮
❯
Specifications
- Immunogen
- Human Vasoactive Intestinal Peptide Receptor 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Human VIPR1
- Species Reactivity
- Human
- Clone
- 18B11
- Applications
- WB, IP, IF, IHC, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- VIPR1; Vasoactive Intestinal Polypeptide Receptor 1; RDC1; PACAP-R2; Vasoactive Intestinal Peptide Receptor 1; PACAP-R-2; Pituitary Adenylate Cyclase-Activating Polypeptide Type II Receptor; VIP-R-1; VIRG; HVR1; VPAC1R; VAPC1; VPCAP1R; VPAC1; V1RG; Pituit
- UniProt ID
- P32241
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Versatile Receptor Antibody
Clone 18B11 is a workhorse for VIPR1 research. We have successfully used it for Western Blot, IP, and Immunofluorescence. It specifically recognizes the human Vasoactive Intestinal Peptide Receptor 1, allowing us to map its cellular distribution accurately.
Robust IHC Staining
We performed Immunohistochemistry with this mouse monoclonal antibody and obtained distinct staining patterns. It helped us visualize the receptor in tissue samples. The antibody is specific and does not seem to cross-react with other GPCRs in our panel.
Q&As
-
What is the specificity and isotype of this antibody?
A: Clone 18B11 is a Mouse IgG2a, kappa monoclonal antibody that recognizes Human VIPR1 (Vasoactive Intestinal Peptide Receptor 1). It binds to amino acids 31-160 of the receptor. It is highly specific for VIPR1 and useful for studying G-protein coupled receptor signaling.
-
Can this antibody be used for Immunofluorescence?
A: Yes, this antibody has been reported for use in Immunofluorescence (IF), as well as Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), and ELISA. It allows researchers to visualize the localization of VIPR1 in various cell types and tissues.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| TAB-359CQ | Anti-Human VIPR1 Recombinant Antibody (hu23A11) | FC, FuncS | Humanized antibody |
| CAT | Product Name | Application | Type |
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| TAB-358CQ-S(P) | Anti-Human VIPR1 Recombinant Antibody scFv Fragment (23A11) | ELISA, FC, WB, Neut |
| CAT | Product Name | Application | Type |
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| TAB-359CQ-S(P) | Anti-Human VIPR1 Recombinant Antibody scFv Fragment (hu23A11) | ELISA, FC, WB, Neut | Humanized antibody |
| CAT | Product Name | Application | Type |
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| TAB-358CQ-F(E) | Anti-Human VIPR1 Recombinant Antibody Fab Fragment (23A11) | ELISA, FC, WB, Neut |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOB-2219CT | Recombinant Mouse anti-Human VIPR1 Monoclonal antibody (BT69) | FC, IHC, WB |
| CAT | Product Name | Application | Type |
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| MHC-LC815 | PE-B*27:05/Human VIPR1 (RRKWRRWHL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC956 | PE-B*27:04/Human VIPR1 (RRKWRRWHL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC959 | PE-B*27:06/Human VIPR1 (RRKWRRWHL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1217 | PE-B*27:09/Human VIPR1 (RRKWRRWHL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC816 | APC-B*27:05/Human VIPR1 (RRKWRRWHL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3840 | Rabbit Anti-VIPR1 Recombinant Antibody (clone DS3840AB) | FC, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1217 | Rabbit Anti-VIPR1 Recombinant Antibody (VS13-YC1217) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC171 | Recombinant Anti-VIPR1 Vesicular Antibody, EV Displayed (VS-0425-YC171) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7858 | Anti-VIPR1 Immunohistochemistry Kit | IHC |
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