Recombinant Mouse Anti-DENV Antibody (PABX-045) (CAT#: PABX-045)

• Immunogen
• Mouse brain DENV

• Host Species
• Mouse

• Derivation
• Mouse

• Type
• IgG

• Specificity
• Tested positive against native DENV

• Species Reactivity
• Dengue virus

• Applications
• WB, ELISA, FuncS, IF

• Purity
• >95% by SDS-PAGE and HPLC analysis

• Storage
• Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

• Application Notes
• The DENV E-DIII antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay, Immunofluorescence.
  For Immunofluorescence: Immunofluorescence staining was performed on infected cells. Cells were probed with mouse anti DV-1 EDIII 4E11 antibody, mouse anti DV-1 HyperImmune Ascitic Fluid or rabbit anti human MHC-II dimer antibodies. Cy3-conjugated goat anti mouse IgG antibody Cy3 conjugated, FITC-conjugated goat anti-mouse IgG antibody, or and FITC-conjugated goat anti-rabbit IgG antibody were used as secondary antibodies respectively.
  For Western Blot: Protein lysates from Vero cells or U937 cells infected with recombinant viruses were fractionated by SDS-PAGE gel electrophoresis and transferred to cellulose membranes. DV1 EDIII (5 ng) produced in drosophila cells was loaded as a positive control. The blots were probed with a murine monoclonal antibody mAb4E11 directed against the E EDIII of DENV1. A goat anti-mouse immunoglobulin G (IgG)-horseradish peroxidase (HRP) conjugate was used as a secondary antibody. Peroxidase activity was visualized with an enhanced chemiluminescence detection kit.

• Alternative Names
• Dengue virus; DENV; dengue virus E-DIII; DENV E-DIII

Select a product category from the dropdown menu below to view related products.