Recombinant Mouse Anti-DENV Antibody (PABX-045) (CAT#: PABX-045)
Anti-DENV antibody is a Mouse antibody of IgG2a κ class that binds to an DENV. This antibody recognize dengue virus, serotype 1 envelope protein.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Specifications
- Immunogen
- Mouse brain DENV
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native DENV
- Species Reactivity
- Dengue virus
- Applications
- WB, ELISA, FuncS, IF
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The DENV E-DIII antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay, Immunofluorescence.
For Immunofluorescence: Immunofluorescence staining was performed on infected cells. Cells were probed with mouse anti DV-1 EDIII 4E11 antibody, mouse anti DV-1 HyperImmune Ascitic Fluid or rabbit anti human MHC-II dimer antibodies. Cy3-conjugated goat anti mouse IgG antibody Cy3 conjugated, FITC-conjugated goat anti-mouse IgG antibody, or and FITC-conjugated goat anti-rabbit IgG antibody were used as secondary antibodies respectively.
For Western Blot: Protein lysates from Vero cells or U937 cells infected with recombinant viruses were fractionated by SDS-PAGE gel electrophoresis and transferred to cellulose membranes. DV1 EDIII (5 ng) produced in drosophila cells was loaded as a positive control. The blots were probed with a murine monoclonal antibody mAb4E11 directed against the E EDIII of DENV1. A goat anti-mouse immunoglobulin G (IgG)-horseradish peroxidase (HRP) conjugate was used as a secondary antibody. Peroxidase activity was visualized with an enhanced chemiluminescence detection kit.
Target
- Alternative Names
- Dengue virus; DENV; dengue virus E-DIII; DENV E-DIII
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Fab Antibody Products
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Mouse Antibody Products
Human Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-275 | Recombinant Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
| MHH-275 | Recombinant Human Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
| PABC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody (1A1D-2) | WB, ELISA | IgG |
| PABL-437 | Recombinant Mouse Anti-DENV EDIII Antibody (2H12) | ELISA, Neut | IgG |
| MRO-1612CQ | Recombinant Human Anti-DENV E-DIII Antibody (DV10) | ELISA, Neut | Human antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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