Recombinant Mouse Anti-DENV Antibody (PABX-045)
CAT#: PABX-045
Anti-DENV antibody is a Mouse antibody of IgG2a κ class that binds to an DENV. This antibody recognize dengue virus, serotype 1 envelope protein.
Specifications
- Immunogen
- Mouse brain DENV
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native DENV
- Species Reactivity
- Dengue virus
- Applications
- WB, ELISA, FuncS, IF
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The DENV E-DIII antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay, Immunofluorescence.
For Immunofluorescence: Immunofluorescence staining was performed on infected cells. Cells were probed with mouse anti DV-1 EDIII 4E11 antibody, mouse anti DV-1 HyperImmune Ascitic Fluid or rabbit anti human MHC-II dimer antibodies. Cy3-conjugated goat anti mouse IgG antibody Cy3 conjugated, FITC-conjugated goat anti-mouse IgG antibody, or and FITC-conjugated goat anti-rabbit IgG antibody were used as secondary antibodies respectively.
For Western Blot: Protein lysates from Vero cells or U937 cells infected with recombinant viruses were fractionated by SDS-PAGE gel electrophoresis and transferred to cellulose membranes. DV1 EDIII (5 ng) produced in drosophila cells was loaded as a positive control. The blots were probed with a murine monoclonal antibody mAb4E11 directed against the E EDIII of DENV1. A goat anti-mouse immunoglobulin G (IgG)-horseradish peroxidase (HRP) conjugate was used as a secondary antibody. Peroxidase activity was visualized with an enhanced chemiluminescence detection kit.
Target
- Alternative Names
- Dengue virus; DENV; dengue virus E-DIII; DENV E-DIII
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "DENV E-DIII"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-275 | Recombinant Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
| MHH-275 | Recombinant Human Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
| PABC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody (1A1D-2) | WB, ELISA | IgG |
| PABL-437 | Recombinant Mouse Anti-DENV EDIII Antibody (2H12) | ELISA, Neut | IgG |
| MRO-1612CQ | Recombinant Human Anti-DENV E-DIII Antibody (DV10) | ELISA, Neut | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-275-F(E) | Recombinant Anti-DENV E-DIII Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| MHH-275-F(E) | Recombinant Human Anti-DENV E-DIII Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
| PFBC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody Fab Fragment (1A1D-2) | WB, ELISA | IgG |
| PFBL-434 | Recombinant Mouse Anti-DENV EDIII Antibody Fab Fragment (2H12) | ELISA, Neut | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-275-S(P) | Recombinant Anti-DENV E-DIII Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
| MHH-275-S(P) | Recombinant Human Anti-DENV E-DIII Antibody scFv Fragment | Neut, IF, FuncS | scFv |
| PSBC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody scFv Fragment (1A1D-2) | WB, ELISA | scFv |
| PSBL-434 | Recombinant Mouse Anti-DENV EDIII Antibody scFv Fragment (2H12) | ELISA, Neut | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-044 | Recombinant Mouse Anti-DENV Antibody (3E31) | WB, ELISA, FuncS, Neut, Inhibt | IgG |
| PABX-054 | Recombinant Mouse Anti-DENV Antibody (E111) | WB, ELISA, FuncS, SPR, Neut | IgG |
| PABX-044-S (P) | Recombinant Mouse Anti-DENV Antibody scFv Fragment (3E31) | WB, ELISA, FuncS | scFv |
| PABX-044-F (E) | Recombinant Mouse Anti-DENV Antibody Fab Fragment (3E31) | WB, ELISA, FuncS | Fab |
| PABX-054-F (E) | Recombinant Mouse Anti-DENV Antibody Fab Fragment (E111) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-054-S (P) | Recombinant Mouse Anti-DENV Antibody scFv Fragment (E111) | WB, ELISA, FuncS | scFv |
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