Mouse Anti-LMAN1 Recombinant Antibody (clone 15A2)
CAT#: MOB-2385z
This product is a mouse antibody that recognizes human LMAN1. It can be used in the following applications: WB, FCM, ICC, IF, IHC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/2063_G6_7_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity with granular pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2320/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_B_8_5.jpg
Normal Tissue
Figure 5 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_B_3_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074695-LMAN1
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Specifications
- Immunogen
- Human Lectin, Mannose-Binding, 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LMAN1
- Species Reactivity
- Human
- Clone
- 15A2
- Applications
- WB, FC, ICC, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- LMAN1; ER-Golgi Intermediate Compartment 53 KDa Protein; Intracellular Mannose-Specific Lectin MR60; Lectin Mannose-Binding 1; F5F8D; ERGIC-53; Coagulation Factor V-Factor VIII Combined Deficiency; Gp58; MR60; Protein ERGIC-53; ERGIC53; Intracellular Mann
- UniProt ID
- P49257
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| BRD-0324MZ | Chicken Anti-LMAN1 Polyclonal IgY | WB | Chicken antibody |
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| MOR-2065 | Hi-Affi™ Recombinant Rabbit Anti-LMAN1 Monoclonal Antibody (DS2065AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY849 | Recombinant Rabbit Anti-LMAN1 Antibody (clone R03-5C3) | WB, IHC-P | Rabbit IgG |
| VS3-XY3418 | Rabbit Anti-LMAN1 Recombinant Antibody (clone R01-8R1) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY307 | Mouse Anti-NHP LMAN1 Recombinant Antibody (clone OTI1A8) | WB, IHC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1251 | Anti-LMAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4049 | Anti-Mouse LMAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4048 | Anti-Human LMAN1 Immunohistochemistry Kit | IHC |
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