Recombinant Mouse Anti-Human MLH1 Antibody (clone FS06)
CAT#: MOB-0328MZ
This antibody is a recombinant mouse monoclonal antibody which specifically reacts with human MLH1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60714/1149_G9_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13294/ihc_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_4_6.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_9_3.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076242-MLH1
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Specifications
- Immunogen
- Recombinant protein corresponding to a 210 amino acid portion of the human MLH1 molecule
- Host Species
- Mouse
- Type
- Mouse antibody
- Specificity
- Human MLH1
- Species Reactivity
- Human
- Clone
- FS06
- Applications
- IHC
Target
REVIEWS AND Q&AS
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Excellent for IHC
The clone FS06 works beautifully for Immunohistochemistry. We were able to stain human tissue sections and clearly visualize MLH1 expression. The specificity for the 210 amino acid portion of the molecule ensures accurate target detection.
Specific to Human MLH1
We tested this mouse monoclonal antibody and found it to be highly specific for human MLH1. The recombinant production provides uniform quality, which is essential for comparing staining intensity across different tumor samples in our mismatch repair studies.
Q&As
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What is the immunogen used for this MLH1 antibody?
A: The immunogen is a recombinant protein corresponding to a 210 amino acid portion of the human MLH1 molecule. This ensures specific recognition of the target protein in your assays.
-
Can this antibody be used for Immunohistochemistry?
A: Yes, this recombinant mouse monoclonal antibody (clone FS06) specifically reacts with human MLH1 and is recommended for use in Immunohistochemistry (IHC) to visualize protein expression in tissues.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MLH1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4013z | Mouse Anti-MLH1 Recombinant Antibody (clone 24D7) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2242 | Hi-Affi™ Recombinant Rabbit Anti-MLH1 Monoclonal Antibody (DS2242AB) | WB, IP, IHC-P, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1005-CN | Recombinant Rabbit Anti-MLH1 Monoclonal Antibody (CBACN-373) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0124F | Mouse Anti-MLH1 Recombinant Antibody (MOB-0124F) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0283C | Mouse Anti-MLH1 Recombinant Antibody (ZG-0283C) | WB, IHC, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0284C | Mouse Anti-MLH1 Recombinant Antibody (ZG-0284C) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY324 | Mouse Anti-NHP MLH1 Recombinant Antibody (clone 4C9C7) | WB, IHC, ICC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC731 | CytoStream™ Rabbit Anti-MLH1 Recombinant Antibody (VS13-YC731) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4417 | Anti-MLH1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4418 | Anti-Rat MLH1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4419 | Anti-Mouse MLH1 Immunohistochemistry Kit | IHC |
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