Recombinant Anti-human EGF Antibody
CAT#: MOB-305
Recombinant Mouse Antibody is capable of binding to Human EGF, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (EFO-21, SK-BR-3, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138798-EGF
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Specifications
- Immunogen
- Human epidermal growth factor
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Human epidermal growth factor
- Species Reactivity
- Human
- Applications
- Antibody assay: Enzyme-linked Immunosorbent Assay; Western blot; Flow Cytometry; Functional Study
Product Property
- Purity
- >98% by SDS-PAGE and HPLC analysis
- Storage
- Store at -20°C. Avoid multiple freeze/thaw cycles.
Target
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Effective Binding
The recombinant anti-human EGF antibody is effective for ELISA, WB, FC, and functional studies. It specifically binds to human epidermal growth factor with high reliability and quality. The product documentation explains its expression in CHO cells and ensures users follow the proper storage protocol to maintain its activity over multiple freeze/thaw cycles.
Consistent Efficacy
I have used this anti-EGF antibody for several experiments and found its binding to be consistent and reliable. The detailed product information, including its use in ELISA and WB, assurances on purity and storage instructions, have all contributed to reproducible results. It is a strong research tool for studying epidermal growth factor functions.
Q&As
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How does the anti-EGF antibody support functional studies?
A: We design the recombinant anti-human EGF antibody to have a high binding affinity to epidermal growth factor, supporting its use in ELISA, WB, FC, and functional assays. Its expression in CHO cells, along with robust quality control through SDS-PAGE and HPLC, assures precise quantification and reproducibility in experiments addressing cell proliferation.
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What storage methods are advised to maintain antibody efficacy?
A: We recommend storing the anti-EGF antibody at 4°C if the entire vial is used within 2-4 weeks, and aliquoting for long-term storage at -20°C. Following these storage methods protects the antibody's high purity and binding effectiveness, ensuring that it remains efficient for ELISA, WB, FC, and other research applications.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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|---|---|---|---|
| MOB-305-F(E) | Recombinant Anti-human EGF Antibody Fab Fragment | IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| MOB-305-S(P) | Recombinant Anti-human EGF Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| CAT | Product Name | Application | Type |
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| MHH-305 | Recombinant Human Anti-human EGF Antibody | ELISA, WB, FuncS | IgG |
| CAT | Product Name | Application | Type |
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| MHH-305-F(E) | Recombinant Human Anti-human EGF Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| MHH-305-S(P) | Recombinant Human Anti-human EGF Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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