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Recombinant Anti-human HSPA1A Antibody Fab Fragment (CAT#: MOB-517-F(E))

Recombinant Mouse Antibody Fab Fragment exhibits selective binding affinity to Human HSPA1A, expressed in Chinese Hamster Ovary cells(CHO).

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Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human testis
Figure 3 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/ihc_selected.jpg
Figure 4 IHC staining of human prostate
Figure 4 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in the basal layer of glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_1_5_selected.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_B_7_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_7_3.jpg
Figure 7 Liver
Figure 7 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_5.jpg
Figure 9 Testis
Figure 9 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_6_6.jpg
Figure 10 Lymph node
Figure 10 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enriched (HEK93)
Figure 11 RNA cell line category: Cell line enriched (HEK93)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204389-HSPA1A

Specifications

  • Immunogen
  • Human heat shock 70kDa protein 1A
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Fab
  • Specificity
  • Tested positive against native Human heat shock 70kDa protein 1A
  • Species Reactivity
  • Human
  • Applications
  • Can be useful in applications such as: Western blot; Radioimmunoassay; Functional Study

Product Property

  • Purity
  • >95% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE
  • Storage
  • ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C

Target

  • Alternative Names
  • HSPA1A; heat shock 70kDa protein 1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103; heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; HSP70.1/HSP70.2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; epididymis s

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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scFv Fragment Antibody Fab Fragment Antibody Blocking Antibody Rabbit Monoclonal Antibody Recombinant Antibody
CAT Product Name Application Type
MOB-517-S(P) Recombinant Anti-human HSPA1A Antibody scFv Fragment ELISA, IF, FuncS scFv
MHH-517-S(P) Recombinant Human Anti-human HSPA1A Antibody scFv Fragment WB, Neut, FuncS scFv
HPAB-0243-YC-S(P) Mouse Anti-HSPA1A Recombinant Antibody (clone cmHsp70.1); scFv Fragment ELISA Mouse scFv
HPAB-0244-YC-S(P) Human Anti-HSPA1A Recombinant Antibody (clone hucmHsp70.1); scFv Fragment ELISA Humanized scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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