Recombinant Anti-human SEMA3E Antibody Fab Fragment
CAT#: MOB-896-F(E)
Recombinant Mouse Antibody Fab Fragment binds specifically to Human SEMA3E, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63804/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HEK93, SK-BR-3, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170381-SEMA3E/subcellular
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Specifications
- Immunogen
- Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Fab
- Specificity
- Tested positive against native Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
- Species Reactivity
- Human
- Applications
- Can be useful in applications such as: Immunoprecipitation; Immunofluorescence; Functional Study
Product Property
- Purity
- >95% by HPLC
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-896 | Recombinant Anti-human SEMA3E Antibody | ELISA, IP, FuncS | IgG |
| MHH-896 | Recombinant Human Anti-human SEMA3E Antibody | Neut, WB, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-896-S(P) | Recombinant Anti-human SEMA3E Antibody scFv Fragment | FC, RIA, Biosensors, FuncS | scFv |
| MHH-896-S(P) | Recombinant Human Anti-human SEMA3E Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-896-F(E) | Recombinant Human Anti-human SEMA3E Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
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