Human Anti-DENV Recombinant Antibody (clone YC090); scFv Fragment (CAT#: DrMAB-937)

Figure 1 DeNV2 NS1-specific HuScFv from bio-panning were testified and produced.

Indirect eLISA results for detecting the binding of HuScFv in lysates of 19 representative huscfv-phagemid transformed E. coli clones to native NS1. Clones no. 11 and 13 (asterisks) were selected for further use as the HuScFv in lysates of these clones gave the highest eLISA signal to the antigen compared with the signal from background (HB) control.

Poungpair, O., Bangphoomi, K., Chaowalit, P., Sawasdee, N., Saokaew, N., Choowongkomon, K., ... & Yenchitsomanus, P. T. (2014, March). Generation of human single-chain variable fragment antibodies specific to dengue virus non-structural protein 1 that interfere with the virus infectious cycle. In mabs (Vol. 6, No. 2, pp. 474-482). Taylor & Francis.

Figure 2 DeNV2 NS1-specific HuScFv from bio-panning were testified and produced.

Patterns of purified HuScFv11 and HuScFv13 were revealed by SDS-PAGe and CBB staining (lanes 1 and 2, respectively).

Poungpair, O., Bangphoomi, K., Chaowalit, P., Sawasdee, N., Saokaew, N., Choowongkomon, K., ... & Yenchitsomanus, P. T. (2014, March). Generation of human single-chain variable fragment antibodies specific to dengue virus non-structural protein 1 that interfere with the virus infectious cycle. In mabs (Vol. 6, No. 2, pp. 474-482). Taylor & Francis.

Figure 3 Immunofluorescent assay demonstrated interaction of HuScFv to native NS1 in DeNV2-infected cells.

Locations of HuScFv11 in Huh-7 cells infected with DeNV2 revealed by confocal microscopy.

Poungpair, O., Bangphoomi, K., Chaowalit, P., Sawasdee, N., Saokaew, N., Choowongkomon, K., ... & Yenchitsomanus, P. T. (2014, March). Generation of human single-chain variable fragment antibodies specific to dengue virus non-structural protein 1 that interfere with the virus infectious cycle. In mabs (Vol. 6, No. 2, pp. 474-482). Taylor & Francis.

Figure 4 NS1 specific-HuScFv reduced DeNV2 virus production from infected cells.

The HuScFv-exposed DeNV-infected cells (MOI = 0.1 and 1.0) had markedly lower virus infectious particles in the culture supernatants at both 24 and 36 h post-treatment in comparison with the control (non-treated) cells.

Poungpair, O., Bangphoomi, K., Chaowalit, P., Sawasdee, N., Saokaew, N., Choowongkomon, K., ... & Yenchitsomanus, P. T. (2014, March). Generation of human single-chain variable fragment antibodies specific to dengue virus non-structural protein 1 that interfere with the virus infectious cycle. In mabs (Vol. 6, No. 2, pp. 474-482). Taylor & Francis.

Figure 5 Mimotope sequences of HuScFv were aligned with the linear sequence of DeNV2 NS1.

The synthetic peptides of epitopes of HuScFv11 (DSGCVVSWKNKeLK) and HuScFv13 (TTNIWWSNVTA) could block the binding of the respective HuScFv to NS1 in the competitive eLISA in dose dependent manner while the irrelevant peptide control (WCDAFCSIrGKrV) did not affect the NS1 binding of HuScFv of both clones.

Poungpair, O., Bangphoomi, K., Chaowalit, P., Sawasdee, N., Saokaew, N., Choowongkomon, K., ... & Yenchitsomanus, P. T. (2014, March). Generation of human single-chain variable fragment antibodies specific to dengue virus non-structural protein 1 that inte protein in infected cells with or without HuScFv treatment determined by cell eLISA were not