Recombinant Human Anti-IgG ch1 domain Antibody Fab Fragment
CAT#: MHH-547-F(E)
Recombinant Human Antibody Fab Fragment recognises an antigen IgG ch1 domain, expressed in HEK 293 cells.
Specifications
- Immunogen
- Immunoglobulin G ch1 domain
- Host Species
- Human
- Derivation
- Human
- Type
- Fab
- Specificity
- Tested positive against native Immunoglobulin G ch1 domain
- Applications
- Used for immunoassaytechniques such as: Western blot; Immunoprecipitation; Functional Study
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.
Target
- Alternative Names
- Immunoglobulin G; IGg; Immunoglobulin G ch1 domain; IgG ch1 domain
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Accurate in ELISA
The specificity of the Recombinant Human Anti-IgG CH1 Domain Antibody Fab Fragment has significantly improved our ELISA assays' accuracy. Its stability across a range of pH levels makes it incredibly versatile for our research.
Minimal cross-reactivity and high affinity
Excellent performance in BCR analysis. The minimal cross-reactivity and high affinity have been critical for our immunotherapy research.
Reliable in IP
We were initially wary of its usage in IP assays due to its monovalent nature, however the specific binding to the CH1 domain has proven quite useful in our application.
Q&As
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Is it possible to employ this Fab fragment of the antibody in ELISA experiments?D141:G151G158D141:G149D141:G153G158D141:G149D141:G155G158D141:G149D141:G157G158D141:G149D141:G159G158D141:G149D141:G161G158D141:G149D141:G163G158D141:G149D141:G164D141:G163
A: Yes, the recombinant Fab fragment is ideal for use in ELISA experiments because of its unique binding to the CH1 domain of human IgG, allowing for precise quantification.
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Is a fluorophore conjugated to the Fab fragment for detection purposes?
A: Our standard offering does not come conjugated. However, customized conjugation with popular fluorophores such as Alexa Fluor and FITC can be requested.
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Can this Fab fragment be used in immunoprecipitation (IP) assays?
A: Fab fragments' high specificity for the CH1 domain makes them valuable for some IP applications, despite the fact that their monovalency normally renders them less effective for IP.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MHH-547-F(E), RRID: AB_3111459)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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See other products for "IgG ch1 domain"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-547 | Recombinant Anti-IgG ch1 domain Antibody | ELISA, WB, FC, FuncS | IgG |
| MHH-547 | Recombinant Human Anti-IgG ch1 domain Antibody | ELISA, WB, Dot, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-547-S(P) | Recombinant Anti-IgG ch1 domain Antibody scFv Fragment | WB, IP, FuncS | scFv |
| MHH-547-S(P) | Recombinant Human Anti-IgG ch1 domain Antibody scFv Fragment | ELISA, WB, Neut, FuncS | scFv |
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