Recombinant Human Anti-human ANPEP Antibody
CAT#: MHH-69
Recombinant Human Antibody reacts with an antigen Human ANPEP, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ANPEP mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-ANPEP mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4625/1612_A1_3_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Microvilli
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_4.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_5_4.jpg
Normal Tissue
Figure 5 Kidney
Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (microvilli)
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, fHDF/TERT166, HMC-1, HSkMC, HUVEC TERT2, TIME, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166825-ANPEP
❮
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Specifications
- Immunogen
- Human alanyl (membrane) aminopeptidase
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human alanyl (membrane) aminopeptidase
- Species Reactivity
- Human
- Applications
- Antibody assay: Western blot; Immunofluorescence; Functional Study
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- ≤1 year at -20°C.
Target
- Alternative Names
- ANPEP; alanyl (membrane) aminopeptidase; APN; CD13; LAP1; P150; PEPN; GP150; aminopeptidase N; AP-M; AP-N; hAPN; aminopeptidase M; alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13
- Gene ID
- 290
- UniProt ID
- A0A024RC61
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Customer Review
There are currently no Customer reviews or questions for MHH-69. Click the button above to contact us or submit your feedback about this product.
Reliable in Western Blot
This antibody detected ANPEP clearly in my Western blots. The bands were sharp at the expected molecular weight. Even with low-abundance samples, the signal was strong after optimization. The datasheet's recommended dilutions were a great starting point. I appreciate the consistency between batches-it saves time troubleshooting.
Great for Immunofluorescence
The antibody produced stunning images in my immunofluorescence assays. It highlighted ANPEP's membrane localization in endothelial cells without cross-reactivity. The staining pattern matched published data, validating its specificity. Easy to use and reliable-exactly what I needed for my project.
Fast Shipping, Well-Packaged
The antibody arrived quickly and was packed securely with ice packs. Despite summer heat, it remained frozen and intact. The speedy delivery let me start experiments without delay. Great service from the supplier!
Q&As
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What is the main target of the Recombinant Human Anti-human ANPEP Antibody?
A: The main target of this antibody is human ANPEP (Alanyl Aminopeptidase), also known as CD13. ANPEP is an enzyme found on the surface of many cell types. It plays roles in breaking down proteins, cell signaling, and immune responses. Researchers use this antibody to study ANPEP's functions in normal and disease-related processes, such as inflammation or cancer, in laboratory settings.
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Does this antibody react with mouse or rat ANPEP?
A: No, this antibody is specific to human ANPEP. It is not designed to detect ANPEP in mice, rats, or other species. Researchers working with non-human samples should verify cross-reactivity or choose a species-specific antibody.
-
Is this antibody suitable for immunofluorescence?
A: Yes! It works well in immunofluorescence. Researchers can use it to visualize ANPEP's location in cells using fluorescent dyes. This helps study how ANPEP moves or interacts with other structures in healthy or diseased cells.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ANPEP"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-69-F(E) | Recombinant Anti-human ANPEP Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-69-F(E) | Recombinant Human Anti-human ANPEP Antibody Fab Fragment | WB, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-69-S(P) | Recombinant Anti-human ANPEP Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| MHH-69-S(P) | Recombinant Human Anti-human ANPEP Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-021CQ | Mouse Anti-ANPEP Recombinant Antibody (clone WM15) | FC, IHC, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0180 | Hi-Affi™ Rabbit Anti-ANPEP Recombinant Antibody (clone DS180AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1003CQ | Human Anti-ANPEP Recombinant Antibody (clone 22A5) | IP, FC, IHC | Human IgG1 |
| VS3-XY51 | Mouse Anti-ANPEP Recombinant Antibody (clone 496CT9.1.1) | WB | Mouse IgG1 |
| VS3-XY52 | Mouse Anti-ANPEP Recombinant Antibody (clone 4A122D1) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY53 | Mouse Anti-ANPEP Recombinant Antibody (clone 9H4C4) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY54 | Mouse Anti-ANPEP Recombinant Antibody (clone 505CT12.1.2) | WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY13 | AbPlus™ Anti-ANPEP Magnetic Beads (CBACN-106) | IP, Protein Purification | |
| VS-0724-YC929 | AbPlus™ Anti-ANPEP Magnetic Beads (VS-0724-YC929) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY75 | Mouse Anti-NHP ANPEP Recombinant Antibody (clone WM15) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC736 | Recombinant Anti-ANPEP Vesicular Antibody, EV Displayed (VS-0425-YC736) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY399 | Anti-Mouse ANPEP Immunohistochemistry Kit | IHC | |
| VS-0525-XY401 | Anti-Human ANPEP Immunohistochemistry Kit | IHC |
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