Recombinant Human Anti-human COL2A1 Antibody (CAT#: MHH-225)
Recombinant Human Antibody molecule has affinity for the Human COL2A1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-COL2A1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-COL2A1 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55753/1472_C7_4_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55753/1472_C7_4_selected.jpg
Figure 2 IHC staining of human cartilage
(Immunohistochemical staining of human cartilage shows moderate cytoplasmic positivity in the chondrocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/2214/1552_866_400x400.jpg
(Immunohistochemical staining of human cartilage shows moderate cytoplasmic positivity in the chondrocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/2214/1552_866_400x400.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2214/169753_B_7_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2214/169753_B_7_5.jpg
Figure 4 RNA cell line category: Cell line enhanced (AF22, HAP1, HEK93)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139219-COL2A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139219-COL2A1
Specifications
- Immunogen
- Human collagen, type II, alpha 1
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human collagen, type II, alpha 1
- Species Reactivity
- Human
- Applications
- Can be useful in applications such as: Western blot; Flow Cytometry; Functional Study
Product Property
- Purity
- >95% as judged by SDS-polyacrylamide gel electrophoresis
- Storage
- ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C
Target
- Alternative Names
- COL2A1; collagen, type II, alpha 1; AOM; ANFH; SEDC; STL1; COL11A3; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; arthroophthalmopathy, progressive (Stickler syndrome)
- Gene ID
- 1280
- UniProt ID
- P02458
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-225-F(E) | Recombinant Anti-human COL2A1 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| MHH-225-F(E) | Recombinant Human Anti-human COL2A1 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| PFBL-644 | Mouse Anti-COL2A1 Recombinant Antibody (clone M2139); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0281-YC-F(E) | Human Anti-COL2A1 Recombinant Antibody; Fab Fragment (HPAB-0281-YC-F(E)) | ELISA, IHC, FuncS | Human Fab |
| PFBC-248 | Mouse Anti-COL2A1 Recombinant Antibody (clone ACC1); Fab Fragment | ELISA, IHC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
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