Recombinant Human Anti-human COL2A1 Antibody Fab Fragment
CAT#: MHH-225-F(E)
Recombinant Human Antibody Fab Fragment recognises an antigen Human COL2A1, expressed in HEK 293 cells.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55753/1472_C7_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cartilage
Immunohistochemical staining of human cartilage shows moderate cytoplasmic positivity in the chondrocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/2214/1552_866_400x400.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2214/169753_B_7_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, HAP1, HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139219-COL2A1
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Specifications
- Immunogen
- Human collagen, type II, alpha 1
- Host Species
- Human
- Derivation
- Human
- Type
- Fab
- Specificity
- Tested positive against native Human collagen, type II, alpha 1
- Species Reactivity
- Human
- Applications
- Products can be used for: Enzyme-linked Immunosorbent Assay; Immunoprecipitation; Functional Study
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- Store at – 20 or -70°Cupon receipt. Divide antibody into aliquots prior usage and avoid multiple freeze-thaw cycles.
Target
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High Specificity
The specificity of this antibody is impressive. It binds specifically to human COL2A1 without detecting other collagen types. In my experiments, this precision has been essential for studying the role of collagen type II in cartilage development. The high specificity has enhanced the accuracy of my results, and I highly recommend this antibody to other researchers.
Highly Recommended
I highly recommend the Recombinant Human Anti-human COL2A1 Antibody Fab Fragment to fellow researchers studying cartilage biology. Its specificity, reliability, and ease of use make it an excellent choice for detecting COL2A1. This antibody has significantly contributed to the progress of my research, and I am very pleased with the results.
Great for ELISA
I used this antibody in ELISA assays to quantify COL2A1 levels in cell culture supernatants. The antibody performed very well, providing sensitive and specific detection. The results were reproducible, and the assay was straightforward to set up. This antibody has been very helpful in measuring collagen production in my experiments.
Q&As
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What is the main target of the Recombinant Human Anti-human COL2A1 Antibody Fab Fragment?
A: The main target of this antibody is human collagen type II alpha 1 (COL2A1) protein. COL2A1 is a vital component of type II collagen, which is primarily found in cartilage tissue and the vitreous humor of the eye. By specifically binding to COL2A1, this antibody is valuable for researchers studying cartilage development, cartilage-related diseases, and extracellular matrix components in various biological processes.
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How should I store the Recombinant Human Anti-human COL2A1 Antibody Fab Fragment?
A: The antibody should be stored at -20°C or lower to maintain its stability and activity. It is recommended to aliquot the antibody into smaller volumes upon first use to avoid repeated freeze-thaw cycles, which can degrade the antibody. Proper storage ensures consistent performance over time.
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How can I use this antibody in ELISA?
A: In ELISA applications, this antibody can be used as a capture or detection antibody to quantify COL2A1 levels in samples such as serum, cell culture supernatants, or tissue extracts. By setting up a standard curve with known concentrations of COL2A1, you can accurately measure its concentration in your samples.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "COL2A1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-225-F(E) | Recombinant Anti-human COL2A1 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| PFBZ-088 | Mouse Anti-COL2A1 Recombinant Antibody (clone ACC4); Fab Fragment | ELISA, IP, IHC | Mouse Fab |
| PFBL-644 | Mouse Anti-COL2A1 Recombinant Antibody (clone M2139); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0281-YC-F(E) | Human Anti-COL2A1 Recombinant Antibody; Fab Fragment (HPAB-0281-YC-F(E)) | ELISA, IHC, FuncS | Human Fab |
| PFBC-248 | Mouse Anti-COL2A1 Recombinant Antibody (clone ACC1); Fab Fragment | ELISA, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-225-S(P) | Recombinant Anti-human COL2A1 Antibody scFv Fragment | WB, FC, FuncS | scFv |
| PSBZ-088 | Mouse Anti-COL2A1 Recombinant Antibody (clone ACC4); scFv Fragment | ELISA, IP, IHC | Mouse scFv |
| PSBL-644 | Mouse Anti-COL2A1 Recombinant Antibody (clone M2139); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0281-YC-S(P) | Human Anti-COL2A1 Recombinant Antibody; scFv Fragment (HPAB-0281-YC-S(P)) | ELISA, IHC, FuncS | Human scFv |
| PSBC-248 | Mouse Anti-COL2A1 Recombinant Antibody (clone ACC1); scFv Fragment | ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0281-YC | Human Anti-COL2A1 Recombinant Antibody (HPAB-0281-YC) | ELISA, IHC, FuncS | Human IgG |
| PABC-248 | Mouse Anti-COL2A1 Recombinant Antibody (clone ACC1) | ELISA, IHC | Mouse IgG |
| MOR-0006-FY | Mouse Anti-COL2A1 Recombinant Antibody (clone M2139) | FuncS, IA | Mouse IgG2b |
| VS3-CJ279 | Mouse Anti-COL2A1 Recombinant Antibody (clone 7F9) | IHC-P | |
| VS3-CJ280 | Mouse Anti-COL2A1 Recombinant Antibody (clone PT0115) | IHC-P | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY501 | Anti-COL2A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1587 | Anti-Mouse COL2A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1588 | Anti-Human COL2A1 Immunohistochemistry Kit | IHC |
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