Recombinant Human Anti-human EGF Antibody
CAT#: MHH-305
Recombinant Human Antibody is capable of binding to Human EGF, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-EGF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-EGF mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (EFO-21, SK-BR-3, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138798-EGF
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Specifications
- Immunogen
- Human epidermal growth factor
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human epidermal growth factor
- Species Reactivity
- Human
- Applications
- Antibody assay: Enzyme-linked Immunosorbent Assay; Western blot; Functional Study
Product Property
- Purity
- >98% by SDS-PAGE and HPLC analysis
- Storage
- Store at -20°C. Avoid multiple freeze/thaw cycles.
Target
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Human EGF Functional Antibody
We are very happy with this reagent. We used it for a functional study (FuncS) to modulate EGF signaling in our human cell culture. Being a recombinant human IgG1 expressed in HEK 293 cells, it was highly compatible with our system and performed as expected.
Reliable for WB/ELISA
This anti-EGF antibody (MHH-305) is a solid performer. We have used it for both Western Blot (WB) and ELISA to detect human EGF. The signal is clean and specific. The HEK 293 expression system is a plus for a human antibody, ensuring proper modifications.
Q&As
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What is the host and expression system for this anti-EGF antibody?
A: This is a recombinant human antibody of the IgG1 isotype. It was produced using the HEK 293 expression system, which is a human cell line. This is often preferred for research applications involving human proteins, as the post-translational modifications, such as glycosylation, are human-like.
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What applications can I use MHH-305 for?
A: This recombinant human antibody is validated for multiple common research applications. Our data confirms its use for Enzyme-linked Immunosorbent Assay (ELISA), Western Blot (WB), and functional studies (FuncS). This makes it a versatile tool for quantifying, detecting, or functionally modulating human EGF.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MHH-305, RRID: AB_3713898)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-305 | Recombinant Anti-human EGF Antibody | ELISA, WB, FC, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-305-F(E) | Recombinant Anti-human EGF Antibody Fab Fragment | IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-305-S(P) | Recombinant Anti-human EGF Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-305-F(E) | Recombinant Human Anti-human EGF Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-305-S(P) | Recombinant Human Anti-human EGF Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-4151 | Hi-Affi™ Rabbit Anti-EGF Recombinant Antibody (clone SI226DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MOR-4152 | Hi-Affi™ Rabbit Anti-EGF Recombinant Antibody (clone SI227DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0157-WJ | Mouse Anti-EGF Recombinant Antibody (clone 0914) | ELISA, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| HPAB-0157-WJ-S(P) | Mouse Anti-EGF Recombinant Antibody (clone 0914); scFv Fragment | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0157-WJ-F(E) | Mouse Anti-EGF Recombinant Antibody (clone 0914); Fab Fragment | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY176 | Mouse Anti-NHP EGF Recombinant Antibody (clone 10827) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0425-YC621 | Recombinant Anti-EGF Vesicular Antibody, EV Displayed (VS-0425-YC621) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
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| VS-0525-XY2180 | Anti-EGF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY2181 | Anti-Mouse EGF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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