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Recombinant Human Anti-human GAPDH Antibody (CAT#: MHH-386)

Recombinant Human Antibody molecule has affinity for the Human GAPDH, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-GAPDH mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-GAPDH mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.


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Specific Inquiry
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/if_selected.jpg
Figure 7 IF staining of human cell line A549
Figure 7 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/1874_F7_61_blue_red_green.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/ihc_selected.jpg
Figure 4 IHC staining of human skin
Figure 4 IHC staining of human skin
(Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_B_8_1_selected.jpg
Figure 5 IHC staining of human kidney
Figure 5 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5197/ihc_selected.jpg
Figure 6 IHC staining of human skeletal muscle
Figure 6 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79968/170623_B_3_6_selected.jpg
Figure 8 Colon
Figure 8 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_3.jpg
Figure 9 Liver
Figure 9 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_4.jpg
Figure 10 Kidney
Figure 10 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_5.jpg
Figure 11 Testis
Figure 11 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_4_6.jpg
Figure 12 Lymph node
Figure 12 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111640-GAPDH

Specifications

  • Immunogen
  • Human glyceraldehyde-3-phosphate dehydrogenase
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human glyceraldehyde-3-phosphate dehydrogenase
  • Species Reactivity
  • Human
  • Applications
  • Can be useful in applications such as: Enzyme-linked Immunosorbent Assay; Western blot; Flow Cytometry; Functional Study

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Storage
  • ≤6 months at 4°C; ≥6 months at -20°C.

Target

  • Alternative Names
  • GAPDH; glyceraldehyde-3-phosphate dehydrogenase; G3PD; GAPD; HEL-S-162eP; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; epididymis secretory sperm binding protein Li 162eP

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "GAPDH"

Recombinant Antibody

CAT Product Name Application Type
MOB-386 Recombinant Anti-human GAPDH Antibody WB, IHC, FuncS IgG
FAMAB-0117YC Human Anti-GAPDH Recombinant Antibody (clone lesion) ELISA, WB Human IgG
MRO-0630-CN Mouse Anti-GAPDH Recombinant Antibody (clone 1-G2) WB, IF Mouse IgG2b
MRO-0631-CN Rabbit Anti-GAPDH Recombinant Antibody (clone CBACN-235) WB, IF, IHC, IP, FC Rabbit IgG
MRO-0632-CN Mouse Anti-GAPDH Recombinant Antibody (clone 5-E10) WB, IF Mouse IgM

Fab Fragment Antibody

CAT Product Name Application Type
MOB-386-F(E) Recombinant Anti-human GAPDH Antibody Fab Fragment IP, RIA, FuncS Fab
MHH-386-F(E) Recombinant Human Anti-human GAPDH Antibody Fab Fragment ELISA, WB, IP, FuncS Fab
FAMAB-0117-YC-F(E) Human Anti-GAPDH Recombinant Antibody (clone lesion); Fab Fragment ELISA, WB Human Fab

scFv Fragment Antibody

CAT Product Name Application Type
MHH-386-S(P) Recombinant Human Anti-human GAPDH Antibody scFv Fragment WB, FC, FuncS scFv
FAMAB-0117-YC-S(P) Human Anti-GAPDH Recombinant Antibody (clone lesion); scFv Fragment ELISA, WB Human scFv

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0217MZ Chicken Anti-GAPDH Polyclonal IgY WB Chicken antibody
BRD-0699MZ Chicken Anti-GAPDH Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1398 Hi-Affi™ Rabbit Anti-GAPDH Recombinant Antibody (clone DS1398AB) WB, ICC, IP, FC Rabbit IgG
MOR-0101-FY Rabbit Anti-GAPDH Recombinant Antibody (clone AFY0072) IF, IHC-P, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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