Recombinant Human Anti-human LGALS1 Antibody
CAT#: MHH-622
Recombinant Human Antibody is directed against Human LGALS1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-LGALS1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-LGALS1 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/687/171_F9_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1130/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human smooth muscle
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/646/1191_B_6_7_selected.jpg
Normal Tissue
Figure 4 IHC staining of human pancreas
Low expression. RNA expression: 155.5 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_2_3_val_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/646/1191_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_4_6.jpg
Normal Tissue
Figure 10 Endometrium
Cells in endometrial stroma
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_B_5_3.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100097-LGALS1
❮
❯
Specifications
- Immunogen
- Human lectin, galactoside-binding, soluble, 1
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Antibody Isotype
- IgG1
- Specificity
- Tested positive against native Human lectin, galactoside-binding, soluble, 1
- Species Reactivity
- Human
- Applications
- Used for immunoassaytechniques such as: Enzyme-linked Immunosorbent Assay; Western blot; Immunofluorescence; Functional Study
Product Property
- Purity
- >95% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE
- Storage
- Store at – 20 or -70°C upon receipt. Divide antibody into aliquots prior usage and avoid multiple freeze-thaw cycles.
Target
- Alternative Names
- LGALS1; lectin, galactoside-binding, soluble, 1; GBP; GAL1; galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-gal
- Gene ID
- 3956
- UniProt ID
- P09382
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-622 | Recombinant Anti-human LGALS1 Antibody | WB, Dot, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-622-F(E) | Recombinant Anti-human LGALS1 Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-622-S(P) | Recombinant Anti-human LGALS1 Antibody scFv Fragment | IF, FC, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-622-F(E) | Recombinant Human Anti-human LGALS1 Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-622-S(P) | Recombinant Human Anti-human LGALS1 Antibody scFv Fragment | WB, IP, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0287CL | Human Anti-LGALS1 Recombinant Antibody (TAB-0287CL) | WB, Neut, IP | Human IgG |
| CAT | Product Name | Application | Type |
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| TAB-0287CL-S(P) | Human Anti-LGALS1 Recombinant Antibody; scFv Fragment (TAB-0287CL-S(P)) | WB, Neut, IP | Human scFv |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-2040 | Hi-Affi™ Recombinant Rabbit Anti-LGALS1 Monoclonal Antibody (DS2040AB) | WB, IP, ICC | IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0140-FY | Mouse Anti-LGALS1 Recombinant Antibody (HPAB-0140-FY) | Inhib, WB, Neut, IHC, ChIP | Mouse IgG2b, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0140-FY-F(E) | Mouse Anti-LGALS1 Recombinant Antibody; Fab Fragment (HPAB-0140-FY-F(E)) | Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0620-CN | Recombinant Rabbit Anti-LGALS1 Monoclonal Antibody (JM13-37) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1326CQ | Mouse Anti-LGALS1 Recombinant Antibody (clone SAIC-25B-112) | Immuno-MRM, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0724-YC1392 | AbPlus™ Anti-LGALS1 Magnetic Beads (VS-0724-YC1392) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0325-XY1239 | Anti-LGALS1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC652 | CytoStream™ Rabbit Anti-LGALS1 Recombinant Antibody (VS13-YC652) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3990 | Anti-Mouse LGALS1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY3991 | Anti-Rat LGALS1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC203 | Recombinant Anti-LGALS1 Eliminating Antibody, pH-Sensitive (VS-0625-YC203) | Antigen-Sweeping In Vivo. | Mouse IgG2b lambda |
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