Recombinant Human Anti-human SEMA3E Antibody
CAT#: MHH-896
Recombinant Human Antibody is directed against Human SEMA3E, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-SEMA3E mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-SEMA3E mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63804/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HEK93, SK-BR-3, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170381-SEMA3E/subcellular
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Specifications
- Immunogen
- Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
- Species Reactivity
- Human
- Applications
- Used for immunoassaytechniques such as: Neutralization; Western blot; Functional Study
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "SEMA3E"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-896 | Recombinant Anti-human SEMA3E Antibody | ELISA, IP, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-896-F(E) | Recombinant Anti-human SEMA3E Antibody Fab Fragment | IP, IF, FuncS | Fab |
| MHH-896-F(E) | Recombinant Human Anti-human SEMA3E Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-896-S(P) | Recombinant Anti-human SEMA3E Antibody scFv Fragment | FC, RIA, Biosensors, FuncS | scFv |
| MHH-896-S(P) | Recombinant Human Anti-human SEMA3E Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
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