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Recombinant Human Anti-human SEMA3E Antibody (CAT#: MHH-896)

Recombinant Human Antibody is directed against Human SEMA3E, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-SEMA3E mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-SEMA3E mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63804/if_selected.jpg
Figure 2 IHC staining of human duodenum
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_B_7_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_8_3.jpg
Figure 5 Liver
Figure 5 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_9_4.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_7_5.jpg
Figure 7 Testis
Figure 7 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29419/61257_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HEK93, SK-BR-3, U-2 OS)
Figure 8 RNA cell line category: Cell line enhanced (HEK93, SK-BR-3, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170381-SEMA3E/subcellular

Specifications

  • Immunogen
  • Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
  • Species Reactivity
  • Human
  • Applications
  • Used for immunoassaytechniques such as: Neutralization; Western blot; Functional Study

Product Property

  • Purity
  • 90–95% (SDS-PAGE)
  • Storage
  • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • SEMA3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; SEMAH; coll-5; M-SEMAH; M-SemaK; semaphorin-3E

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "SEMA3E"

Recombinant Antibody

CAT Product Name Application Type
MOB-896 Recombinant Anti-human SEMA3E Antibody ELISA, IP, FuncS IgG

Fab Fragment Antibody

CAT Product Name Application Type
MOB-896-F(E) Recombinant Anti-human SEMA3E Antibody Fab Fragment IP, IF, FuncS Fab
MHH-896-F(E) Recombinant Human Anti-human SEMA3E Antibody Fab Fragment ELISA, WB, IP, FuncS Fab

scFv Fragment Antibody

CAT Product Name Application Type
MOB-896-S(P) Recombinant Anti-human SEMA3E Antibody scFv Fragment FC, RIA, Biosensors, FuncS scFv
MHH-896-S(P) Recombinant Human Anti-human SEMA3E Antibody scFv Fragment ELISA, WB, IF, FuncS scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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