Recombinant Human Anti-human VIP Antibody
CAT#: MHH-1041
Recombinant Human Antibody binds specifically to Human VIP, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-VIP mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-VIP mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72701/1760_E4_3_cr594b84e510be2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in islet cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17324/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17324/44727_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes - Microvilli
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17324/44727_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17324/44727_A_8_4.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17324/44727_A_2_3.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (SH-SY5Y, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000146469-VIP/subcellular
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Specifications
- Immunogen
- Human vasoactive intestinal peptide
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human vasoactive intestinal peptide
- Species Reactivity
- Human
- Applications
- Used for immunoassaytechniques such as: Immunofluorescence; Functional Study
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "VIP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1041 | Recombinant Anti-human VIP Antibody | WB, FC, FuncS | IgG |
| MOB-0978MZ | Recombinant Mouse Anti-Human VIP Antibody (clone 44) | ELISA | Mouse antibody |
| HPAB-0091-CN | Mouse Anti-VIP Recombinant Antibody (clone c23.5) | ELISA | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1041-F(E) | Recombinant Anti-human VIP Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-1041-F(E) | Recombinant Human Anti-human VIP Antibody Fab Fragment | WB, FC, FuncS | Fab |
| HPAB-0091-CN-F(E) | Mouse Anti-VIP Recombinant Antibody (clone c23.5); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1041-S(P) | Recombinant Anti-human VIP Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-1041-S(P) | Recombinant Human Anti-human VIP Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| HPAB-0091-CN-S(P) | Mouse Anti-VIP Recombinant Antibody (clone c23.5); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3839 | Rabbit Anti-VIP Recombinant Antibody (clone DS3839AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1124-YJ12 | Human Anti-VIP Recombinant Autoantibody (clone hkl3) | ELISA, Catalytic | Human Light chain |
| VS-1124-YJ13 | Human Anti-VIP Recombinant Autoantibody (clone hkl4) | ELISA, Catalytic | Human Light chain |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7857 | Anti-VIP Immunohistochemistry Kit | IHC |
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