Mouse Anti-CD3E Recombinant Antibody (clone UCHT1); Fab Fragment (CAT#: DrMAB-989)

Recombinant Mouse Antibody binds selectively to Mouse CD3E.


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FC

Figure 1 Concanavalin A expanded γδ T cells staining

Figure 1 Concanavalin A expanded γδ T cells staining

Concanavalin A expanded γδ T cells were stained with UCHT1, Fab, Fabred, or left untreated, followed by an APC-labeled anti-mouse IgG antibody as a secondary reagent. Fluorescence intensities were quantified by flow cytometry (n > 3).

Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunogy, 9, 1579.

IF

Figure 2 Close proximity between the TCR and Nck was detected by in situ PLA.

Figure 2 Close proximity between the TCR and Nck was detected by in situ PLA.

Zoledronate-expanded γδ T cells were either left untreated (−) or treated with 5 µg/ml UCHT1 in the absence or presence of 10 nM AX-024 at 37°C for 5 min. After fixation and permeabilization, PLA was performed using the primary antibodies goat anti-CD3ε (M20) and rabbit anti-Nck1, and the corresponding secondary antibodies. Nuclei were stained with DAPI.

Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunogy, 9, 1579.

Cyt

Figure 3 UCHT1 increased tumor killing by γδ T cells.

Figure 3 UCHT1 increased tumor killing by γδ T cells.

51Cr-labeled Daudi and Raji cells were incubated with zoledronate-expanded human γδ T cells without (−) or with the addition of 5 µg/ml UCHT1 using an effector to target (T cell to tumor cell) ratio of 12.5:1.

Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunology, 9, 1579.

Stim

Figure 4 Using its SH3.1(Nck) domain Nck binds to the UCHT1-stimulated γδ T cell antigen receptor (TCR).

Figure 4 Using its SH3.1(Nck) domain Nck binds to the UCHT1-stimulated γδ T cell antigen receptor (TCR).

Zoledronate-expanded γδ T cells were left untreated (−) or stimulated with 5 µg/ml UCHT1 at 37°C for 5 min

Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunology, 9, 1579.

PP

Figure 5 Preparation of UCHT1 Fab fragments.

Figure 5 Preparation of UCHT1 Fab fragments.

The anti-CD3ε antibody UCHT1 was digested with papain using a kit from Thermo Fisher Scientific. The Fab fragments were purified with protein A coupled beads.

Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunogy, 9, 1579.

Agonist

Figure 6 Fab and Fabred fragments enhanced tumor killing by γδ T cells.

Figure 6 Fab and Fabred fragments enhanced tumor killing by γδ T cells.

51Cr-labeled Daudi and Raji cells were incubated with zoledronate or with 5 µg/ml UCHT1, 3.33 µg/ml Fab, or 3.33 µg/ml Fabred (these concentrations were chosen to obtain equimolar amounts of the different reagents). The effector to target ratio was 12.5:1.

Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunogy, 9, 1579.


Specifications

  • Immunogen
  • Human thymocytes/Sezary T cells
  • Host Species
  • Mouse
  • Type
  • Mouse Fab
  • Specificity
  • Antibody reacts the 20 kd epsilon chain of the CD3 molecule complex.
  • Species Reactivity
  • Human
  • Clone
  • UCHT1
  • Applications
  • FC, Cyt, Stim, PP, Agonist

Product Property

  • Purification
  • Protein A purification
  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Concentration
  • 1 mg/ml
  • Buffer
  • 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The CD3E antibody has been reported in applications of Flow Cytometry, Cytotoxicity, Stimulation, Protein Purification, Agonist.
    Cyt: 5 µg/ml.
    Stim: 5 µg/ml .

Target

  • Alternative Names
  • CD3; CD3 epsilon; CD3e molecule, epsilon (CD3-TCR complex); TCRE; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rat Antibody

CAT Product Name Application Type
TAB-H28 Anti-Human CD3E Recombinant Antibody (Ertumaxomab) ELISA, FC, IP, FuncS, IF, Neut, ICC IgG2a / G2b

Humanized Antibody

CAT Product Name Application Type
TAB-714 Anti-CD3E Recombinant Antibody (Visilizumab) FuncS, IF, Neut, ELISA, FC, IP, ICC IgG2
TAB-H52 Anti-Human CD3E Recombinant Antibody (Otelixizumab) FC, IP, ELISA, Neut, FuncS, IF, WB IgG1 - lambda
TAB-420CQ Anti-Human CD3E Recombinant Antibody (Visilizumab) ELISA, IHC, FC, IP, IF, FuncS IgG2, κ

Human Antibody

CAT Product Name Application Type
TAB-245 Anti-Human CD3 epsilon Recombinant Antibody (Foralumab) WB, ELISA, FC, IP, FuncS, IF, Neut IgG1 - kappa
TAB-124CL Human Anti-CD3E Recombinant Antibody (TAB-124CL) ELISA Human IgG

Mouse Antibody

Immunotoxin

CAT Product Name Application Type
AGTO-G010E Anti-CD3E immunotoxin UCHT1 (scFv)-PE Cytotoxicity assay, Function study
AGTO-G010D Anti-CD3E immunotoxin UCHT1 (scFv)-DT Cytotoxicity assay, Function study
AGTO-G010R Anti-CD3E immunotoxin UCHT1 (scFv)-RTA Cytotoxicity assay, Function study
AGTO-L049E anti-CD3E immunotoxin UCHT1 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L049D anti-CD3E immunotoxin UCHT1 (IgG)-DT Cytotoxicity assay, Functional assay

Rabbit Monoclonal Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-245 Afuco™ Anti-CD3E ADCC Recombinant Antibody (Foralumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF ADCC enhanced antibody
AFC-TAB-H52 Afuco™ Anti-CD3E ADCC Recombinant Antibody (Otelixizumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody
AFC-TAB-714 Afuco™ Anti-CD3E ADCC Recombinant Antibody (Visilizumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-019 Afuco™ Anti-CD3E ADCC Recombinant Antibody (Muromonab-Cd3), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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