Recombinant Mouse Antibody binds selectively to Mouse CD3E.
Figure 1 Concanavalin A expanded γδ T cells staining
Concanavalin A expanded γδ T cells were stained with UCHT1, Fab, Fabred, or left untreated, followed by an APC-labeled anti-mouse IgG antibody as a secondary reagent. Fluorescence intensities were quantified by flow cytometry (n > 3).
Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunogy, 9, 1579.
Figure 2 Close proximity between the TCR and Nck was detected by in situ PLA.
Zoledronate-expanded γδ T cells were either left untreated (−) or treated with 5 µg/ml UCHT1 in the absence or presence of 10 nM AX-024 at 37°C for 5 min. After fixation and permeabilization, PLA was performed using the primary antibodies goat anti-CD3ε (M20) and rabbit anti-Nck1, and the corresponding secondary antibodies. Nuclei were stained with DAPI.
Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunogy, 9, 1579.
Figure 3 UCHT1 increased tumor killing by γδ T cells.
51Cr-labeled Daudi and Raji cells were incubated with zoledronate-expanded human γδ T cells without (−) or with the addition of 5 µg/ml UCHT1 using an effector to target (T cell to tumor cell) ratio of 12.5:1.
Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunology, 9, 1579.
Figure 4 Using its SH3.1(Nck) domain Nck binds to the UCHT1-stimulated γδ T cell antigen receptor (TCR).
Zoledronate-expanded γδ T cells were left untreated (−) or stimulated with 5 µg/ml UCHT1 at 37°C for 5 min
Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunology, 9, 1579.
Figure 5 Preparation of UCHT1 Fab fragments.
The anti-CD3ε antibody UCHT1 was digested with papain using a kit from Thermo Fisher Scientific. The Fab fragments were purified with protein A coupled beads.
Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunogy, 9, 1579.
Figure 6 Fab and Fabred fragments enhanced tumor killing by γδ T cells.
51Cr-labeled Daudi and Raji cells were incubated with zoledronate or with 5 µg/ml UCHT1, 3.33 µg/ml Fab, or 3.33 µg/ml Fabred (these concentrations were chosen to obtain equimolar amounts of the different reagents). The effector to target ratio was 12.5:1.
Juraske, C., Wipa, P., Morath, A., Hidalgo, J. V., Hartl, F. A., Raute, K., ... & Pongcharoen, S. (2018). Anti-CD3 Fab fragments enhance tumor killing by human γδ T cells independent of Nck recruitment to the γδ T cell antigen receptor. Frontiers in immunogy, 9, 1579.
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• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H28 | Anti-Human CD3E Recombinant Antibody (Ertumaxomab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG2a / G2b |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-714 | Anti-CD3E Recombinant Antibody (Visilizumab) | FuncS, IF, Neut, ELISA, FC, IP, ICC | IgG2 |
TAB-H52 | Anti-Human CD3E Recombinant Antibody (Otelixizumab) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG1 - lambda |
TAB-420CQ | Anti-Human CD3E Recombinant Antibody (Visilizumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG2, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-245 | Anti-Human CD3 epsilon Recombinant Antibody (Foralumab) | WB, ELISA, FC, IP, FuncS, IF, Neut | IgG1 - kappa |
TAB-124CL | Human Anti-CD3E Recombinant Antibody (TAB-124CL) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-019 | Anti-Human CD3 Recombinant Antibody (Muromonab-CD3) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG2a - kappa |
PABX-032-S (P) | Recombinant Grey hamster Anti-CD3E Antibody scFv Fragment (2C11) | WB, ELISA, FuncS | scFv |
PABX-033-S (P) | Recombinant Grey hamster Anti-CD3E Antibody scFv Fragment (17A2) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G010E | Anti-CD3E immunotoxin UCHT1 (scFv)-PE | Cytotoxicity assay, Function study | |
AGTO-G010D | Anti-CD3E immunotoxin UCHT1 (scFv)-DT | Cytotoxicity assay, Function study | |
AGTO-G010R | Anti-CD3E immunotoxin UCHT1 (scFv)-RTA | Cytotoxicity assay, Function study | |
AGTO-L049E | anti-CD3E immunotoxin UCHT1 (IgG)-PE | Cytotoxicity assay, Functional assay | |
AGTO-L049D | anti-CD3E immunotoxin UCHT1 (IgG)-DT | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-648 | Mouse Anti-CD3E Recombinant Antibody (clone 2C11) | WB, IHC, IF, FuncS | Mouse IgG |
HPAB-0263-YJ | Rabbit Anti-CD3E Recombinant Antibody (clone 645) | ELISA, FC, FuncS | Rabbit IgG1 |
ZG-0863F | Mouse Anti-CD3E Recombinant Antibody (clone M2AB) | FC | Mouse IgG1 |
VS3-FY285 | Recombinant Mouse Anti-CD3E Antibody (clone 8D6-6C1-3B2) | IHC-P | Mouse IgG2b |
VS3-FY286 | Recombinant Rabbit Anti-CD3E Antibody (clone R02-1B5) | WB, IHC-P, IP | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-643 | Mouse Anti-CD3E Recombinant Antibody (clone 2C11); scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
FAMAB-0049-YC-S(P) | Hamster Anti-CD3E Recombinant Antibody (clone 145-2C11); scFv Fragment | FC, FuncS | Hamster scFv |
FAMAB-0052-YC-S(P) | Hamster Anti-CD3E Recombinant Antibody (clone 500A2); scFv Fragment | ELISA | Hamster scFv |
FAMAB-0047CQ-S(P) | Mouse Anti-CD3E Recombinant Antibody (clone CLB-T3/4.1); scFv Fragment | ELISA, IF, IP | Mouse scFv |
HPAB-0347-YJ-S(P) | Rat Anti-CD3E Recombinant Antibody (clone 1Ab); scFv Fragment | FC, WB, Inhib | Rat scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-032 | Recombinant Grey hamster Anti-CD3E Antibody (2C11) | WB, ELISA, FuncS, IP | IgG |
PABX-033 | Recombinant Grey hamster Anti-CD3E Antibody (17A2) | WB, ELISA, FuncS | IgG |
PABX-032-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (2C11) | WB, ELISA, FuncS | Fab |
PABX-033-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (17A2) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0558 | Hi-Affi™ Rabbit Anti-CD3E Recombinant Antibody (clone DS558AB) | IHC-P | Rabbit IgG |
MOR-0058-FY | Rabbit Anti-CD3E Recombinant Antibody (clone AFY0029) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0049-YC-F(E) | Hamster Anti-CD3E Recombinant Antibody (clone 145-2C11); Fab Fragment | FC, FuncS | Hamster Fab |
FAMAB-0052-YC-F(E) | Hamster Anti-CD3E Recombinant Antibody (clone 500A2); Fab Fragment | ELISA | Hamster Fab |
HPAB-0263-YJ-F(E) | Rabbit Anti-CD3E Recombinant Antibody (clone 645); Fab Fragment | ELISA, FC, FuncS | Rabbit Fab |
HPAB-0347-YJ-F(E) | Rat Anti-CD3E Recombinant Antibody (clone 1Ab); Fab Fragment | FC, WB, Inhib | Rat Fab |
HPAB-0385-FY-F(E) | Human Anti-CD3E Recombinant Antibody; Fab Fragment (HPAB-0385-FY-F(E)) | Activ | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-245 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Foralumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-H52 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Otelixizumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-714 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Visilizumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
AFC-TAB-019 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Muromonab-Cd3), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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