Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment

Name: Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment
SKU: DrMAB-1035
Rating: 5 (1 reviews)

CAT#: DrMAB-1035

Recombinant Mouse Antibody binds selectively to Mouse HSPD1, expressed in E. coli.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg

Subcellular Location Figure 3 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg

Subcellular Location Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg

Subcellular Location Figure 5 IHC staining of human colon

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg

Subcellular Location Figure 6 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg

Subcellular Location Figure 7 Cerebral cortex

Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg

Normal Tissue Figure 8 Colon

Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg

Normal Tissue Figure 10 Pancreas

Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg

Normal Tissue Figure 11 Kidney

Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg

Normal Tissue Figure 12 Testis

Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg

RNA Expression Figure 13 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1

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Specifications

Host Species
Mouse

Type
Mouse Fab

Specificity
Human HSPD1

Species Reactivity
Human

Clone
YC181

Applications
ELISA

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
50 mM Potassium Phosphate, 10 mM Boric Acid, 150 mM NaCl, pH 7

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Range of detection of recommended pairing: 0.1 - 1200 ng/mL

Target

Alternative Names
HSPD1; heat shock 60kDa protein 1 (chaperonin); heat shock 60kD protein 1 (chaperonin), spastic paraplegia 13 (autosomal dominant), SPG13; 60 kDa heat shock protein, mitochondrial; GROEL; HSP60; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60

Gene ID
3329

UniProt ID
A0A024R3X4

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - DrMAB-1035.pdf

MSDS

MSDS - DrMAB-1035.pdf

COA

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