Recombinant Mouse Anti-Human MMP9 Antibody Fab Fragment
CAT#: DrMAB-1048
Recombinant Mouse Antibody binds selectively to Mouse MMP9, expressed in E. coli.
Published Data
Gene Expression
WB
Figure 1 MMP-9, MMP-2, TIMP-1, and TIMP-2 protein levels were measured using western blot analysis.
Qin, W., Li, J., Zhu, R., Gao, S., Fan, J., Xia, M., ... & Zhang, J. (2019). Melatonin protects blood-brain barrier integrity and permeability by inhibiting matrix metalloproteinase-9 via the NOTCH3/NF-κB pathway. Aging (Albany NY), 11(23), 11391.
IF
Figure 2 Melatonin pretreatment ameliorates the effect of MMP-9 on the expression of VE-cadherin, occludin, claudin-5, and ZO-1 in the BBB in different treatment groups.
Qin, W., Li, J., Zhu, R., Gao, S., Fan, J., Xia, M., ... & Zhang, J. (2019). Melatonin protects blood-brain barrier integrity and permeability by inhibiting matrix metalloproteinase-9 via the NOTCH3/NF-κB pathway. Aging (Albany NY), 11(23), 11391.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Type
- Fab
- Specificity
- Does not recognize the 67kDa Active form of MMP-9, but does recognize the 82kDa Active form of MMP-9 and pro-MMP-9
- Species Reactivity
- Human
- Applications
- LMNX, ELISA, WB, IF
Product Property
- Purity
- >95% (SDS-PAGE)
- Concentration
- 1 to 25 mg/mL (lot-specific)
- Buffer
- 50 mM Potassium Phosphate, 10 mM Boric Acid, 150 mM NaCl, pH 7
- Storage
- 2-8°C short term, or <-15°C short or long term. Avoid freeze-thaw cycles
Applications
- Application Notes
- The Human MMP9 antibody has been reported in applications of LMNX, Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
Target
- Alternative Names
- MMP9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); CLG4B, matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase), matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa typ
- Gene ID
- 4318
- UniProt ID
- P14780
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-533CL | Anti-Human MMP9 Recombinant Antibody (TAB-533CL) | WB, Inhib | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0824CL | Human Anti-MMP9 Recombinant Antibody (TAB-0824CL) | ELISA | Human IgG4 |
| TAB-0825CL | Human Anti-MMP9 Recombinant Antibody (TAB-0825CL) | ELISA | Human IgG4 |
| TAB-0824CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0824CL-S(P)) | ELISA | Human scFv |
| TAB-0825CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0825CL-S(P)) | ELISA | Human scFv |
| TAB-0824CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0824CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0826CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0826CL) | Inhib, ELISA, IHC | Mouse IgG |
| TAB-0827CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0827CL) | ELISA | Mouse IgG |
| TAB-0826CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0826CL-S(P)) | ELISA | Mouse scFv |
| TAB-0827CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0827CL-S(P)) | ELISA | Mouse scFv |
| TAB-0826CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0826CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-174 | Mouse Anti-MMP9 Recombinant Antibody Fab Fragment (PFBC-174) | ELISA, Neut | Mouse Fab |
| HPAB-0199-FY-F(E) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); Fab Fragment | ELISA, FC | Human Fab, λ |
| HPAB-0200-FY-F(E) | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0513LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); Fab Fragment | FC | Mouse Fab |
| HPAB-0514LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); Fab Fragment | FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0717MZ | Chicken Anti-MMP9 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1748CQ | Recombinant Mouse Anti-MMP9 Antibody (VMC204) | Inhib, IHC-Fr, IP, IHC-P, WB, IHC, ICC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2263 | Hi-Affi™ Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (DS2263AB) | FC, IHC-P, WB | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002ML | Anti-Human MMP9 Recombinant Antibody (TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0027-YC-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (HPAB-0027-YC-S(P)) | FC | Mouse scFv |
| HPAB-0028-YC-S(P) | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1); scFv Fragment | Neut | Human scFv |
| HPAB-0513LY-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); scfv Fragment | FC | Mouse scfv |
| HPAB-0514LY-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); scfv Fragment | FC | Mouse scfv |
| HPAB-1320-FY-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (HPAB-1320-FY-F(E)) | Activ | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1014-CN | Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (JA80-73) | WB, IF, IHC, FC | Rabbit IgG |
| VS-0522-LC77 | Mouse Anti-MMP9 Recombinant Antibody (VS-0522-LC77) | WB, IF, IHC | Mouse IgG2a |
| VS-0522-LC78 | Rat Anti-MMP9 Recombinant Antibody (VS-0522-LC78) | WB, IF, IHC | Rat IgG2b |
| VS-0522-LC79 | Human Anti-MMP9 Recombinant Antibody (VS-0522-LC79) | WB, IF, IHC | Human IgG1 |
| VS-0522-LC80 | Rabbit Anti-MMP9 Recombinant Antibody (VS-0522-LC80) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-002ML | Afuco™ Anti-MMP9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX783 | Mouse Anti-MMP9 Recombinant Antibody (clone 331CT17.4.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY101 | Human Anti-MMP9 (clone DX-2802) scFv-Fc Chimera | WB, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1357 | Anti-MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4460 | Anti-Mouse MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4461 | Anti-Zebrafish MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4459 | Anti-Human MMP9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC735 | CytoStream™ Rabbit Anti-MMP9 Recombinant Antibody (VS13-YC735) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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