Recombinant Rat Anti-mouse Itgam Antibody, clone 5C6
CAT#: DrMAB-104
Recombinant Rat Antibody binds selectively to Rat Itgam.
Published Data
Gene Expression
WB
Figure 1 Macrophages from WT, Clec7a−/−, Clec4n−/− , and Itgam−/− mice were stimulated with or without (0 min) H. capsulatum (MOI = 5) for 30 and 60 min.
Huang, J. H., Liu, C. Y., Wu, S. Y., Chen, W. Y., Chang, T. H., Kan, H. W., ... & Wu-Hsieh, B. A. (2018). NLRX1 facilitates Histoplasma capsulatum-induced LC3-associated phagocytosis for cytokine production in macrophages. Frontiers in immunology, 9, 2761.
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Normal Tissue
Figure 1 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2274/158194_A_8_8.jpg
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Specifications
- Immunogen
- Fusion of spleen cells from AO rats immunised with Thioglycollate-elicted muine peritoneal macrophages (TPM) and the Y3 rat myeloma line.
- Host Species
- Rat
- Type
- IgG
- Specificity
- This antibody specifically recognize CD11b, also known as the integrin alpha M chain (MAC-1), which is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
- Clone
- 5C6
- Applications
- IF, IHC-Fr, WB
Product Property
- Purification
- Protein A affinity purified
- Buffer
- PBS with preservative (0.02% Proclin 300)
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Applications
- Application Notes
- The mouse Itgam antibody has been reported in applications of Immunofluorescence, Immunohistochemistry-Frozen, Western Blot.
Target
- Alternative Names
- Itgam; integrin alpha M; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik; integrin alpha-M; CD11 antigen-like family member B; CD11B (p170); CR-3 alpha chain; Mac-1 alpha; cell surface glycoprotein MAC-1 alpha subunit; cell surface
- Gene ID
- 16409
- UniProt ID
- E9Q604
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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