Recombinant Human Anti-HIV-1 Antibody Fab Fragment (447-52D)
CAT#: PABX-088-F (E)
Anti-HIV-1 antibody is a Human of Fab class that binds to an HIV-1. This antibody recognizes the hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary isolates, making it one of the most effective anti-V3 antibodies characterized to date.
Published Data
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Figure 1 Binding of the mutated 447-52D IgG to the indicated gp141 trimers as measured by ELISA.
McGuire, A. T., Glenn, J. A., Lippy, A., & Stamatatos, L. (2014). Diverse recombinant HIV-1 Envs fail to activate B cells expressing the germline B cell receptors of the broadly neutralizing anti-HIV-1 antibodies PG9 and 447-52D. Journal of virology, 88(5), 2645-2657.
-2.png)
Figure 2 The area under the curve of the Ca2 flux response (determined in panel C) through the mutated 447-52D BCR correlates with the affinity of sIgG 447-53D (determined in panel A).
McGuire, A. T., Glenn, J. A., Lippy, A., & Stamatatos, L. (2014). Diverse recombinant HIV-1 Envs fail to activate B cells expressing the germline B cell receptors of the broadly neutralizing anti-HIV-1 antibodies PG9 and 447-52D. Journal of virology, 88(5), 2645-2657.
-3.png)
Figure 3 Neutralization of chimeric psVs by antibodies 447-52D (purple) and 2220 (blue).
The psV consist of the SF162 strain with its V3 loop replaced by the indicated V3 loop sequence. Consensus B-R18Q is a psV consisting of the consensus subtype B V3 loop sequence with position 18 in the V4 loop mutated from a subtype B consensus arginine t
Almond, D., Krachmarov, C., Swetnam, J., Zolla-Pazner, S., & Cardozo, T. (2012). Resistance of subtype C HIV-1 strains to anti-V3 loop antibodies. Advances in virology, 2013.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- Fab
- Specificity
- Tested positive against native HIV-1
- Species Reactivity
- Human immunodeficiency virus 1
- Clone
- 447-52D
- Applications
- WB 1:1000-1:5000
ELISA 1:10000-1:20000
IF 1:100 -1:500
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- HIV-1; human immunodeficiency virus
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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