Recombinant Human Anti-HIV-1 Antibody Fab Fragment (PGT145) (CAT#: PABX-098-F (E))

Anti-HIV-1 antibody is a Human of Fab class that binds to an HIV-1.

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Figure 4 Effect of GPI-CDR H3(PG16) on anti-HIV-1 activity of transduced human CD5 T cells.

(A) GPI-CDR H3(PG16) confers resistance to HIV-1 Bru-3 in human CD4 T cells. (B) GPI-CDR H3(PG16) confers resistance to HIV-1 Bru-Yu2 in human CD5 T cells.

Liu, L., Wen, M., Wang, W., Wang, S., Yang, L., Liu, Y., ... & Zhou, P. (2011). Potent and broad anti-HIV-1 activity exhibited by a glycosyl-phosphatidylinositol-anchored peptide derived from the CDR H3 of broadly neutralizing antibody PG16. Journal of viȔ

Figure 1 Binding kinetics for BG505.SOSIP and 15-sc gp140 with VRC26.09 and PGT146.

Georgiev, I. S., Joyce, M. G., Yang, Y., Sastry, M., Zhang, B., Baxa, U., ... & Schön, A. (2015). Single-chain soluble BG505. SOSIP gp140 trimers as structural and antigenic mimics of mature closed HIV-1 Env. Journal of virology, 89(10), 5318-5330.

Figure 2 Binding of SOSIP and SOSIP-sfGFP proteins in HEK293T cell supernatant to bNAbs 2G12 and PGT146 as assessed by ELISA.

SOSIP and SOSIP-sfGFP proteins were captured onto the solid phase.

Sliepen, K., Van Montfort, T., Ozorowski, G., Pritchard, L., Crispin, M., Ward, A., & Sanders, R. (2015). Engineering and characterization of a fluorescent native-like HIV-1 envelope glycoprotein trimer. Biomolecules, 5(4), 2919-2934.

Figure 3 Coomassie-stained reducing and non-reducing SDS-PAGE gels showing SOSIP and SOSIP-sfGFP, both purified with a PGT146-affinity column.

Sliepen, K., Van Montfort, T., Ozorowski, G., Pritchard, L., Crispin, M., Ward, A., & Sanders, R. (2015). Engineering and characterization of a fluorescent native-like HIV-1 envelope glycoprotein trimer. Biomolecules, 5(4), 2919-2934.

Figure 4 Antigenicity characterization by ECLIA. An extended panel of both broadly and poorly neutralizing antibodies was assessed using ECLIA for cleaved (black open circles) and 15-sc gp140 (red filled circles) BG506.SOSIP constructs.

Sliepen, K., Van Montfort, T., Ozorowski, G., Pritchard, L., Crispin, M., Ward, A., & Sanders, R. (2015). Engineering and characterization of a fluorescent native-like HIV-1 envelope glycoprotein trimer. Biomolecules, 5(4), 2919-2934.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • Fab
  • Specificity
  • Tested positive against native HIV-1
  • Species Reactivity
  • Human immunodeficiency virus 1
  • Clone
  • PGT145
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    FuncS (assay-depended)

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • HIV-1; human immunodeficiency virus

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "HIV-1"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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