Z-domain of protein A scaffold protein anti-Human amyloid beta (Aβ) peptides (CAT#: SZA-L227)

This human amyloid beta (Aβ) peptides specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=17nM) for human amyloid beta (Aβ) peptides as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.