Z-domain of protein A scaffold protein anti-Human HER3

Name: Z-domain of protein A scaffold protein anti-Human HER3
SKU: SZA-L224
Rating: 5 (1 reviews)

CAT#: SZA-L224

This HER3 specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=0.7 nM) for HER3 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70524/162441_A_4_2_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_8_5_selected.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/659_B11_4_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/658_B11_1_blue_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_7_5.jpg

Normal Tissue Figure 8 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_5.jpg

Normal Tissue Figure 11 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_4_6.jpg

Normal Tissue Figure 12 Skin

Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Langerhans
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_8_1.jpg

Normal Tissue Figure 13 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_8.jpg

RNA Expression Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000065361-ERBB3

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Specifications

Scaffold Name
Z-domain of protein A

Origin
Staphylococcal protein A

Core Structure
3-α helixes

Variable Regions
13 Residues on first and second helix surface

Target
HER3

Species Reactivity
Human

Expression Host
E. coli

Affinity Constant
0.7 nM

Applications
ELISA; IHC; Microscopy; FC; WB; FuncS

Target

Alternative Names
Staphylococcal protein A; Z-domain of protein A; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3

Gene ID
2065

UniProt ID
P21860

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

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Datasheet

Datasheet - SZA-L224.pdf

MSDS

MSDS - SZA-L224.pdf

COA

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