Z-domain of protein A scaffold protein anti-Human TNFα

Name: Z-domain of protein A scaffold protein anti-Human TNFα
SKU: SZA-L241
Rating: 5 (1 reviews)

CAT#: SZA-L241

This TNFα specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=0.1–0.5 nM) for TNFα as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

Gene Expression

Normal Tissue Figure 1 Tonsil

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF

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Specifications

Scaffold Name
Z-domain of protein A

Origin
Staphylococcal protein A

Core Structure
3-α helixes

Variable Regions
13 Residues on first and second helix surface

Target
TNFα

Species Reactivity
Human

Expression Host
E. coli

Affinity Constant
0.1–0.5 nM

Applications
ELISA; IHC; Microscopy; FC; WB; FuncS

Target

Alternative Names
Staphylococcal protein A; Z-domain of protein A; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha

Gene ID
7124

UniProt ID
P01375

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Related Diseases

Asthma

Inflammatory Bowel Diseases

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

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Datasheet

Datasheet - SZA-L241.pdf

MSDS

MSDS - SZA-L241.pdf

COA

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