FITC-A*02:01/Human PLAC1 (SIDWFMVTV) MHC Tetramer
CAT#: MHC-CN1139
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/163/1910_E11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining shows high expression in placenta
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63425/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63425/141568_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63425/141568_A_7_5.jpg
Normal Tissue
Figure 5 Placenta
Cytotrophoblasts
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63425/141568_A_1_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEK93, hTCEpi, hTERT-HME1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170965-PLAC1/subcellular
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Specifications
- Allele
- A*02:01
- Class
- Class I
- MHC Species
- Human
- Antigen
- PLAC1
- Antigen Species
- Human
- Peptide
- SIDWFMVTV
- Conjugate
- FITC
- Application
- FCM, IHC
Target
REVIEWS AND Q&AS
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Customer Review
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Easy Conjugation Options
The option to choose different fluorophores for the MHC Pentamer was a plus, allowing me to customize my panel depending on the experimental needs.
Cost-Effective Solution
The price point for this MHC Pentamer is very reasonable considering the quality and reliability of the results. It's an excellent value for the cost.
High Binding Avidity
The pentamer's design ensures high binding avidity, which improved my detection limits substantially. Noticeable improvement compared to tetramers I used before.
Q&As
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Can I use this tetramer for detecting the protein in different cell lines?
A: The PLAC1 peptide that HLA-A*02:01 presents is recognized by certain T cells, which is how the tetramer is intended to identify them. Direct protein identification in cell lines is not done using it.
-
Are there known limitations to using this tetramer?
A: HLA restriction and PLAC1 peptide specificity are two drawbacks. It might not be relevant to people who do not express the peptide or who are not HLA-A*02:01.
-
What methods are available for conjugating the tetramer with detection agents?
A: FITC is commonly pre-conjugated using tetramers. Chemical cross-linking techniques such as biotin-streptavidin interactions can be used if further conjugation is required.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PLAC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1977z | Mouse Anti-PLAC1 Recombinant Antibody (clone 41B2) | WB, ELISA, IF, IHC, IP | Mouse IgG1, κ |
| MOB-088YJL | Mouse Anti-PLAC1 Recombinant Antibody (clone CBP91) | WB, IP, IF, IHC-P, ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1137 | PE-A*02:01/Human PLAC1 (SIDWFMVTV) MHC Tetramer | FCM, IHC | |
| MHC-CN1138 | APC-A*02:01/Human PLAC1 (SIDWFMVTV) MHC Tetramer | FCM, IHC | |
| MHC-CN1140 | A*02:01/Human PLAC1 (SIDWFMVTV) MHC Tetramer | FCM, IHC | |
| MHC-YF661 | A*02:01/Human PLAC1 (SIDWFMVTV) MHC Pentamer | FCM |
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