aFGF-PE immunotoxin
CAT#: AGTO-L016E
This immunotoxin was achieved by conjugating/fusing the aFGF to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to epidermoid carcinoma cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of epidermoid carcinoma.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67204/1419_D10_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67204/1382_D10_2_blue_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4231/11838_B_7_5.jpg
Normal Tissue
Figure 4 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4231/11838_A_6_6.jpg
Normal Tissue
Figure 5 Skin
Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4231/11838_B_8_1.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (CACO-2, HaCaT, Hep G2, NTERA-2, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000068078-FGFR3
❮
❯
Specifications
- Host Species
- Human
- Species Reactivity
- Human
- Molecule Class
- aFGF-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Epidermoid carcinoma
Target
- Alternative Names
- FGFR3; fibroblast growth factor receptor 3; ACH; CEK2; JTK4; CD333; HSFGFR3EX; FGFR-3; tyrosine kinase JTK4; hydroxyaryl-protein kinase; fibroblast growth factor receptor 3 variant 4; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35
- Gene ID
- 2261
- UniProt ID
- P22607
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for AGTO-L016E. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Bladder Cancer
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "FGFR3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1249z | Mouse Anti-FGFR3 Recombinant Antibody (clone 3F6) | WB, ELISA, FC | Mouse IgG2a |
| PABL-474 | Human Anti-FGFR3 Recombinant Antibody (clone R3Mab) | ELISA | Human IgG |
| HPAB-AP654-YC | Human Anti-FGFR3 Recombinant Antibody (HPAB-AP654-YC) | ELISA, Inhib, FuncS | Human IgG |
| HPAB-AP655-YC | Human Anti-FGFR3 Recombinant Antibody (HPAB-AP655-YC) | ELISA, Inhib, FuncS | Human IgG |
| HPAB-AP656-YC | Human Anti-FGFR3 Recombinant Antibody (HPAB-AP656-YC) | ELISA, Inhib, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G028E | Anti-FGFR3 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G028G | Anti-FGFR3 immunotoxin (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-L016G | aFGF-Gel immunotoxin | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-095 | Human Anti-FGFR3 Recombinant Antibody (clone 2B.1.3); scFv Fragment | ELISA, WB, IF, FuncS | Human scFv |
| PFBW-162 | Human Anti-FGFR3 Recombinant Antibody (clone R3); Fab Fragment | Neut, ELISA, FC | Human Fab |
| HPAB-AP654-YC-S(P) | Human Anti-FGFR3 Recombinant Antibody; scFv Fragment (HPAB-AP654-YC-S(P)) | ELISA, Inhib, FuncS | Human scFv |
| HPAB-AP655-YC-S(P) | Human Anti-FGFR3 Recombinant Antibody; scFv Fragment (HPAB-AP655-YC-S(P)) | ELISA, Inhib, FuncS | Human scFv |
| HPAB-AP656-YC-S(P) | Human Anti-FGFR3 Recombinant Antibody; scFv Fragment (HPAB-AP656-YC-S(P)) | ELISA, Inhib, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-062WM | Mouse Anti-FGFR3 Recombinant Antibody (TAB-062WM) | ELISA, FC, Neut, WB | Mouse IgG |
| TAB-063WM | Mouse Anti-FGFR3 Recombinant Antibody (TAB-063WM) | ELISA, FC, Neut, WB | Mouse IgG |
| TAB-064WM | Anti-Human FGFR3 Recombinant Antibody (15D8) | Neut, ELISA | |
| TAB-062WM-F(E) | Mouse Anti-FGFR3 Recombinant Antibody; Fab Fragment (TAB-062WM-F(E)) | ELISA, FC, Neut, WB | Mouse Fab |
| TAB-063WM-F(E) | Mouse Anti-FGFR3 Recombinant Antibody; Fab Fragment (TAB-063WM-F(E)) | ELISA, FC, Neut, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-834CQ | Mouse Anti-FGFR3 Recombinant Antibody (clone CBL546) | Neut | Mouse IgG1 |
| NEUT-835CQ | Mouse Anti-FGFR3 Recombinant Antibody (clone CBL547) | WB, Neut | Mouse IgG1 |
| NEUT-836CQ | Mouse Anti-FGFR3 Recombinant Antibody (clone 6G11) | WB, Neut | Mouse IgG1 |
| NEUT-837CQ | Mouse Anti-FGFR3 Recombinant Antibody (clone CBL061) | WB, IHC, Neut | Mouse IgG1 |
| NEUT-838CQ | Rat Anti-Fgfr3 Recombinant Antibody (clone CBL548) | Neut | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1299 | Hi-Affi™ Rabbit Anti-FGFR3 Recombinant Antibody (clone DS1299AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0157-YC-F(E) | Human Anti-FGFR3 Recombinant Antibody; Fab Fragment (HPAB-0157-YC-F(E)) | ELISA, IP, WB | Human Fab |
| HPAB-0158-YC-F(E) | Mouse Anti-FGFR3 Recombinant Antibody; Fab Fragment (HPAB-0158-YC-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0159-YC-F(E) | Human Anti-FGFR3 Recombinant Antibody (clone Antibody 1); Fab Fragment | ELISA, FuncS | Human Fab |
| HPAB-0160-YC-F(E) | Human Anti-FGFR3 Recombinant Antibody (clone PRO-001); Fab Fragment | FC, FuncS | Human Fab |
| HPAB-0161-YC-F(E) | Human Anti-FGFR3 Recombinant Antibody (clone PRO-055); Fab Fragment | FC, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY838 | Anti-FGFR3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2544 | Anti-Mouse FGFR3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2543 | Anti-Human FGFR3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC387 | Recombinant Anti-FGFR3 Vesicular Antibody, EV Displayed (VS-0425-YC387) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC82 | Recombinant Anti-FGFR3 (Domain 2 x Domain 3c) Biparatopic Antibody, Tandem scFv (Clone PRO-001 x Clone PRO-055) | FC | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
-3.png)
Application: ELISA, FC, Block, SPR
-3-1.png)
Application: Neut, Inhib, FC, ELISA
Application: ELISA, FC
Application: ELISA, Neut
Application: WB, FC, IF, Inhib, ELISA, IHC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
