Human Anti-40 kDa glycoprotein Recombinant Antibody (clone NR-LU-13) (CAT#: HPAB-S0001-YC)
Provided is a chimeric antibody NR-LU-13. This antibody contains the murine Fv region of NR-LU-10 and therefore comprises the same binding specificity as NR-LU-10. NR-LU-10 is a nominal 150 kilodalton molecular weight murine IgG2b pancarcinoma monoclonal antibody that recognizes an approximately 40 kilodalton glycoprotein antigen expressed on most carcinomas. Thus, this chimeric antibody binds the NR-LU-10 antigen.
Specific Inquiry
ELISA
Figure 1 Competitive immunoreactivity profile in which the huNR-LU-13 antibody (△) and muNR-LU-10 antibody (■) was allowed to compete with a peroxidase-labeled muNR-LU-10 antibody for binding to an 0.1% NP40 antigen extract from the LS-174T human colon adenocarcinoma cell line.
The huNR-LU-13 antibody exhibited an immunoreactivity profile that was indistinguishable from the muNR-LU-10 antibody.
Graves, S. S., Goshorn, S. C., Stone, D. M., Axworthy, D. B., Reno, J. M., Bottino, B., ... & Libby, R. T. (1999). Molecular modeling and preclinical evaluation of the humanized NR-LU-13 antibody. Clinical Cancer Research, 5(4), 899-908.
Figure 2 N-Linked muNR-LU-10 and huNR-LU-13 carbohydrate analysis after glycosidic digestion and FACE electrophoresis.
Lanes 1 and 8, glucose polymers from partial wheat starch digest; Lanes 2 and 5, PNGaseF 1 NANaseIII glycosidic digestions; Lanes 3 and 6, PNGaseF glycosidic digestion; Lanes 4 and 7, PNGaseF 1 b -galactosidase digestions. The two antibodies contain virtually identical profiles in terms of band migration and general appearance. An exception is the slowest migrating N-linked oligosaccharide, greatly reduced in the humanized version, at approximately the G8 position.
Graves, S. S., Goshorn, S. C., Stone, D. M., Axworthy, D. B., Reno, J. M., Bottino, B., ... & Libby, R. T. (1999). Molecular modeling and preclinical evaluation of the humanized NR-LU-13 antibody. Clinical Cancer Research, 5(4), 899-908.
Figure 3 Evaluation of cytotoxic effector cell function of the muNRLU-10 and huNR-LU-10 antibodies via 51Cr-release assay.
A, ADCC with either muNR-LU-10 or the huNR-LU-13 antibody and human peripheral blood mononuclear cells for 3.5 h. B, compliment-mediated cytotoxicity analysis of either the muNR-LU-10 or huNR-LU-13 antibody and human serum for 3.5 h. Open symbols, MCF-7 cells; closed symbols, SW1222 cells; circles, muNR-LU-10 antibody; squares, huNR-LU-13 antibody.
Graves, S. S., Goshorn, S. C., Stone, D. M., Axworthy, D. B., Reno, J. M., Bottino, B., ... & Libby, R. T. (1999). Molecular modeling and preclinical evaluation of the humanized NR-LU-13 antibody. Clinical Cancer Research, 5(4), 899-908.
Figure 4 Biodistribution of the muNR-LU-10 and huNRLU-13 antibodies in athymic nude mice bearing the s.c.SW1222 human tumor xenograft.
Equimolar quantities of 131I-muNR-LU-10 and 125I-NRLU-13 antibody were i.v. coinjected, and animals were sacrificed at 4, 24, 48, and 192 h after injection. A, biodistribution represented as the percentage of injected dose/gram of tissue. BL, blood; TA, tail; SK, skin; MU, muscle; BO, bone; LU, lung; LV, liver; SP, spleen; ST, stomach; KD, kidney; INT, intestine; TU, tumor. B, 131I-muNR-LU-10 and 125IhuNR-LU-13 tumor: blood ratio analyzed through 192 h after injection.
Graves, S. S., Goshorn, S. C., Stone, D. M., Axworthy, D. B., Reno, J. M., Bottino, B., ... & Libby, R. T. (1999). Molecular modeling and preclinical evaluation of the humanized NR-LU-13 antibody. Clinical Cancer Research, 5(4), 899-908.
Specifications
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric (mouse/human) IgG
- Specificity
- Human 40 kDa glycoprotein
- Species Reactivity
- Human
- Clone
- NR-LU-13
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Human cancer antigen
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0001-YC-F(E) | Human Anti-40 kDa glycoprotein Recombinant Antibody (clone NR-LU-13); Fab Fragment | ELISA | Chimeric (mouse/human) Fab |
| HPAB-S0002-YC-F(E) | Human Anti-40 kDa glycoprotein Recombinant Antibody (clone NRX451); Fab Fragment | ELISA, ADCC | Humanized Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0001-YC-S(P) | Human Anti-40 kDa glycoprotein Recombinant Antibody (clone NR-LU-13); scFv Fragment | ELISA | Mouse scFv |
| HPAB-S0002-YC-S(P) | Human Anti-40 kDa glycoprotein Recombinant Antibody (clone NRX451); scFv Fragment | ELISA, ADCC | Humanized scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0002-YC | Human Anti-40 kDa glycoprotein Recombinant Antibody (clone NRX451) | ELISA, ADCC | Humanized IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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