Human Anti-A-beta oligomer Recombinant Antibody (clone W8) (CAT#: HPAB-J0015-YC)
The antibody can be specifically bound with the A-beta oligomer, effectively inhibit the fibrosis aggregation of A-beta and obviously alleviate the toxic effect of the A-beta on cells.
Specific Inquiry
WB
Figure 1 Western-blot analyses of purified scFv antibodies and characterization of binding of scFv’s to Aβ oligomers.
ScFv antibodies were transferred to nitrocellulose membrane and probed with 9E10 antibody. Bound antibodies were detected using a HRP-conjugated goat anti-mouse and visualized with DAB.
Wang, X. P., Zhang, J. H., Wang, Y. J., Feng, Y., Zhang, X., Sun, X. X., ... & Zhao, M. (2009). Conformation-dependent single-chain variable fragment antibodies specifically recognize beta-amyloid oligomers. FEBS letters, 583(3), 579-584.
DB
Figure 2 Western-blot analyses of purified scFv antibodies and characterization of binding of scFv’s to Aβ oligomers.
Aggregation of A42 was monitored by dot-blot. Aliquots of samples were applied to nitrocellulose membrane (0.12 μg/dot) at the time indicated and then probed with 4G8 and scFv antibodies, respectively.
Wang, X. P., Zhang, J. H., Wang, Y. J., Feng, Y., Zhang, X., Sun, X. X., ... & Zhao, M. (2009). Conformation-dependent single-chain variable fragment antibodies specifically recognize beta-amyloid oligomers. FEBS letters, 583(3), 579-584.
FuncS
Figure 3 Effects of scFv antibodies on fibrillation of Aβ42.
Effects of W8, W9, WC2 on fibrillation of Aβ42. ThT fluorescence was measured after 28 h incubation of 10 μM Ab42 in the absence or presence of scFv's.
Wang, X. P., Zhang, J. H., Wang, Y. J., Feng, Y., Zhang, X., Sun, X. X., ... & Zhao, M. (2009). Conformation-dependent single-chain variable fragment antibodies specifically recognize beta-amyloid oligomers. FEBS letters, 583(3), 579-584.
Inhib
Figure 4 Effects of scFv antibodies on Aβ42-induced cytotoxicity.
Aliquots of Aβ42 preincubated with or without scFv's at indicated times were added to wells coated with SH-SY5Y cells. The final concentration of both Aβ42 and scFv was μlM. After an additional two-day incubation of cells and samples, the viability of the cells was measured using both MTT toxicity assay
Wang, X. P., Zhang, J. H., Wang, Y. J., Feng, Y., Zhang, X., Sun, X. X., ... & Zhao, M. (2009). Conformation-dependent single-chain variable fragment antibodies specifically recognize beta-amyloid oligomers. FEBS letters, 583(3), 579-584.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human A-beta oligomer
- Species Reactivity
- Human
- Clone
- W8
- Applications
- WB, DB, FuncS, Inhib, ELISA
- Related Disease
- Alzheimer's disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The A-beta oligomer antibody has been reported in applications of Western Blot, Dot Blot, Functional Assay, Inhibition, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- Amyloid beta; A beta; Abeta; Aβ
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0012-YC | Human Anti-A-beta oligomer Recombinant Antibody (clone W20) | WB, DB, FuncS, Inhib, ELISA | Human IgG |
| HPAB-J0013-YC | Human Anti-A-beta oligomer Recombinant Antibody (clone W20-1) | ELISA, DB, Inhib | Human IgG |
| HPAB-J0014-YC | Human Anti-A-beta oligomer Recombinant Antibody (clone W20-2) | ELISA, DB, Inhib | Human IgG |
| HPAB-J0016-YC | Human Anti-A-beta oligomer Recombinant Antibody (clone W8-1) | ELISA, DB, Inhib | Human IgG |
| HPAB-J0017-YC | Human Anti-A-beta oligomer Recombinant Antibody (clone W8-2) | ELISA, DB, Inhib | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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