Mouse Anti-A2M Recombinant Antibody (clone 3-G5)
CAT#: MRO-0078-CN
This antibody is a mouse monoclonal antibody that is capable of binding to A2M.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human liver
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2265/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows distinct positivity in blood plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17621/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2265/7607_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2265/7607_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2265/7607_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2265/7607_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2265/7607_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (AF22, ASC diff, Hep G2, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175899-A2M
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG2c
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- 3-G5
- Applications
- WB, IHC, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Clone 3G5 Specificity
We selected Clone 3G5 for our research on protease inhibitors in plasma. The Anti-A2M Recombinant Antibody specifically recognizes Alpha-2-Macroglobulin and does not cross-react with other abundant plasma proteins in our Western Blot analysis. This specificity allowed us to quantify A2M levels accurately in our experimental samples. The clear bands and lack of smearing indicate a high-quality preparation that is free of degrading contaminants.
Great for Protein Analysis
Our lab uses this antibody for immunoprecipitation of A2M complexes. It binds the target efficiently, allowing us to isolate the protein and analyze its bound proteases via mass spectrometry. The recombinant format is a huge plus, as it guarantees consistent binding kinetics from experiment to experiment. We have recommended this product to our collaborators who are also interested in the alpha-macroglobulin family.
Q&As
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I am looking for a specific clone for Alpha-2-Macroglobulin. Can you confirm the clone ID for this product and its target?
A: Thank you for your query. We can confirm that this Recombinant Anti-A2M Antibody is derived from Clone 3G5. It is designed to specifically target Alpha-2-Macroglobulin (A2M), a large plasma protein that functions as a broad-spectrum protease inhibitor. This clone has been selected for its binding properties and is available in a recombinant format for your research needs.
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What species is this antibody raised in, or is it a recombinant construct?
A: This is a Recombinant Antibody, meaning it is produced using recombinant DNA technology rather than being raised in a live animal host in the traditional sense. However, the sequence is typically derived from a source species (often Mouse or Rabbit) and expressed in a host system. This ensures high purity and consistency compared to traditional monoclonal or polyclonal antibodies.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-0589MZ | Mouse Anti-A2M Recombinant Antibody (clone 3E0) | ELISA, IHC-P, WB | Mouse IgG1 |
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| MOR-0002 | Hi-Affi™ Rabbit Anti-A2M Recombinant Antibody (clone DS2AB) | IHC-P, WB | Rabbit IgG |
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| MRO-0015-CN | Rabbit Anti-A2M Recombinant Antibody (clone JM81-41) | WB, IHC | Rabbit IgG |
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| MRO-0079-CN | Mouse Anti-A2M Recombinant Antibody (clone A5-A9) | WB, IHC, ELISA | Mouse IgG1 |
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| MRO-0080-CN | Mouse Anti-A2M Recombinant Antibody (clone 8-B10) | WB, IHC, ELISA | Mouse IgG1 |
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| MRO-0081-CN | Mouse Anti-A2M Recombinant Antibody (clone B1-D7) | WB, IHC, ELISA | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
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