Mouse Anti-ABCA4 Recombinant Antibody (clone Rim 3F4)
CAT#: FAMAB-1014CQ
This product is a recombinant mouse antibody that recognizes ABCA4. The antibody was purified by affinity chromatography. It can be used in IP, WB, ELISA, IF, IHC.
Published Data
Gene Expression
WB
Figure 1 Western blots of ROS proteins labeled with a monoclonal and polyclonal antibody to the 220-kDa protein.
Solubilized ROS membranes (lane a) and the unbound DEAE fraction (lane b) were subjected to SDS-gel electrophoresis (10 ug of protein/lane). Gels were stained with Coomassie Blue (CB) and Western blots were labeled with antibodies to the 220-kDa protein (monoclonal antibody Rim 3F4 or polyclonal antibody PrimT1) and monoclonal antibodies to the 230-kDa Na/Ca-K exchanger (PMe 2D9) and the 240-kDa b subunit of the cGMP-gated channel (PMs 4B2) for comparison. The 220-kDa protein was present in both fractions; the exchanger and channel were only present in the ROS membrane fraction.
Illing, M., Molday, L. L., & Molday, R. S. (1997). The 220-kDa rim protein of retinal rod outer segments is a member of the ABC transporter superfamily. Journal of Biological Chemistry, 272(15), 10303-10310.
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Normal Tissue
Figure 1 Low expression in pancreas
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78826/167673_A_3_3_val_selected.jpg
Normal Tissue
Figure 2 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25% Proximal tubules
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78826/167673_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78826/167673_A_4_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (BEWO, EFO-21, HeLa, SK-BR-3, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198691-ABCA4
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Specifications
- Immunogen
- Rim ABC protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Bovine ABCA4
- Species Reactivity
- Bovine, Xenopus laevis, Xenopus tropicalis, Human, Mouse
- Clone
- Rim 3F4
- Applications
- IP, WB, ELISA, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ATP Binding Cassette Subfamily A Member 4; ATP-Binding Cassette, Sub-Family A (ABC1), Member 4; ATP-Binding Cassette Transporter, Retinal-Specific; ATP-Binding Cassette Sub-Family A Member 4; Stargardt Disease Protein; RIM ABC Transporter; RIM Protein; ABCR; RMP; Retinal-Specific ATP-Binding Cassette Transporter; ATP-Binding Transporter, Retina-Specific; ATP Binding Cassette Transporter
- Gene ID
- 24
- UniProt ID
- P78363
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Reliable for Long-term Projects
This antibody has been used for extended projects, and its effectiveness has always been dependable. It is a vital part of our research instrumentation.
Detailed Protocol Guidance
We needed less troubleshooting to get started quickly because to the comprehensive protocol instruction that was given. The paperwork included all the information we required in easy-to-read detail.
High Signal Intensity
High signal intensity was obtained in our experiments, which aided in precise quantification. This has been essential to the accomplishment of our current research.
Q&As
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What are the advantages of using recombinant antibodies?
A: Comparing recombinant antibodies to conventional monoclonal or polyclonal antibodies, the former have lower variability, higher specificity/purity, and increased batch consistency.
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What steps should I take if my antibody isn't producing expected results?
A: Review the protocol's procedures, adjust antibody concentrations, make sure the reagents are stored properly, and confirm that all of the reagents are working properly to troubleshoot. See technical support if problems continue.
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What is the best way to ensure the antibody's stability over time?
A: Stability can be increased by aliquoting the antibody, reducing freeze-thaw cycles, and maintaining ideal storage conditions. When the manufacturer recommends using stabilizing agents, do so.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone Rim 3F4"
- CAT
- Product Name
See other products for "ABCA4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC735 | Recombinant Anti-ABCA4 Vesicular Antibody, EV Displayed (VS-0425-YC735) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY13 | Anti-Mouse ABCA4 Immunohistochemistry Kit | IHC |
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