Mouse Anti-ABCA4 Recombinant Antibody (clone Rim 3F4) (CAT#: FAMAB-1014CQ)
This product is a recombinant mouse antibody that recognizes ABCA4. The antibody was purified by affinity chromatography. It can be used in IP, WB, ELISA, IF, IHC.
Specific Inquiry
WB
Figure 1 Western blots of ROS proteins labeled with a monoclonal and polyclonal antibody to the 220-kDa protein.
Solubilized ROS membranes (lane a) and the unbound DEAE fraction (lane b) were subjected to SDS-gel electrophoresis (10 ug of protein/lane). Gels were stained with Coomassie Blue (CB) and Western blots were labeled with antibodies to the 220-kDa protein (monoclonal antibody Rim 3F4 or polyclonal antibody PrimT1) and monoclonal antibodies to the 230-kDa Na/Ca-K exchanger (PMe 2D9) and the 240-kDa b subunit of the cGMP-gated channel (PMs 4B2) for comparison. The 220-kDa protein was present in both fractions; the exchanger and channel were only present in the ROS membrane fraction.
Illing, M., Molday, L. L., & Molday, R. S. (1997). The 220-kDa rim protein of retinal rod outer segments is a member of the ABC transporter superfamily. Journal of Biological Chemistry, 272(15), 10303-10310.
Specifications
- Immunogen
- Rim ABC protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Bovine ABCA4
- Species Reactivity
- Bovine, Xenopus laevis, Xenopus tropicalis, Human, Mouse
- Clone
- Rim 3F4
- Applications
- IP, WB, ELISA, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ATP Binding Cassette Subfamily A Member 4; ATP-Binding Cassette, Sub-Family A (ABC1), Member 4; ATP-Binding Cassette Transporter, Retinal-Specific; ATP-Binding Cassette Sub-Family A Member 4; Stargardt Disease Protein; RIM ABC Transporter; RIM Protein; ABCR; RMP; Retinal-Specific ATP-Binding Cassette Transporter; ATP-Binding Transporter, Retina-Specific; ATP Binding Cassette Transporter
- Gene ID
- 24
- UniProt ID
- P78363
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Customer Reviews and Q&As
Customer Review
Q&As
Reliable for Long-term Projects
This antibody has been used for extended projects, and its effectiveness has always been dependable. It is a vital part of our research instrumentation.
Detailed Protocol Guidance
We needed less troubleshooting to get started quickly because to the comprehensive protocol instruction that was given. The paperwork included all the information we required in easy-to-read detail.
High Signal Intensity
High signal intensity was obtained in our experiments, which aided in precise quantification. This has been essential to the accomplishment of our current research.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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