Mouse Anti-ABCB1 Recombinant Antibody (clone 5C12D3) (CAT#: VS4-WK2)
This product is a mouse IgG2b antibody that recognizes human ABCB1. The 5C12D3 antibody is recommended for WB (1/1000-1/5000), IF (1/50-1/200) and FC (1/50-1/200).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
Figure 6 RNA cell line category: Cell line enhanced (Hep G2, RH-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
Specifications
- Immunogen
- Recombinant Human Calnexin protein (350-710AA)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human ABCB1
- Species Reactivity
- Human
- Clone
- 5C12D3
- Applications
- ELISA, WB, IF, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS with 50% Glycerol, pH 7.3.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C. Avoid repeated freeze.
- Shipping
- Ice packs
Applications
- Application Notes
- Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, FC:1:50-1:200
Target
- Alternative Names
- ATP Binding Cassette Subfamily B Member 1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; Multidrug Resistance Protein 1; Colchicin Sensitivity; P-Glycoprotein 1; EC 3.6.3.44; MDR1; PGY1; ATP-Binding Cassette Sub-Family B Member 1; Doxorubicin Resistance;
- Gene ID
- 5243
- UniProt ID
- P08183
- Long Name
- ATP Binding Cassette Subfamily B Member 1
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22 | Recombinant Anti-human ABCB1 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-22 | Recombinant Human Anti-human ABCB1 Antibody | FC, Neut, Funcs | IgG |
| MOB-1496CT | Mouse Anti-ABCB1 Recombinant Antibody (clone 5F4) | FC, IF | Mouse IgG2a |
| PABC-444 | Human Anti-ABCB1 Recombinant Antibody (PABC-444) | FuncS, FC, Block, IP, IHC | Human IgG1, κ |
| HPAB-0076-YJ | Mouse Anti-ABCB1 Recombinant Antibody (HPAB-0076-YJ) | IF, FC, IHC-P, ELISA | Mouse IgG2a, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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