Mouse Anti-ABCB1 Recombinant Antibody (HPAB-0076-YJ)
CAT#: HPAB-0076-YJ
This product is a mouse antibody that recognizes a mapped epitope in the extracellular domain of human P-glycoprotein. The mAb is useful for the identification or purification of cells which express human Pgp, e.g. when contained in heterogeneous cell populations, and for monitoring the multi-drug resistant status of cells, e.g. tumor cells. This antibody can be used as a P-Glycoprotein inhibitor and functions in modulating cancer multidrug resistance.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Hep G2, RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
❮
❯
Specifications
- Immunogen
- Living intact human multidrug-resistant cells
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Human ABCB1
- Species Reactivity
- Human
- Applications
- IF, FC, IHC-P, ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody MM4.17 has been reported in applications of Immunofluorescence, Flow Cytometry, Immunohistochemistry-Paraffin, Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Flow Cytometry: MAb MM4.17 can be used at a concentration of 10 μg/mL.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for HPAB-0076-YJ. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ABCB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22 | Recombinant Anti-human ABCB1 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-22 | Recombinant Human Anti-human ABCB1 Antibody | FC, Neut, Funcs | IgG |
| PABC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219) | WB, IF, IHC, FC, FuncS | Mouse IgG |
| PABC-444 | Human Anti-ABCB1 Recombinant Antibody (PABC-444) | FuncS, FC, Block, IP, IHC | Human IgG1, κ |
| VS3-QX2 | Mouse Anti-ABCB1 Recombinant Antibody (clone 1G6) | WB, IP, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-F(E) | Recombinant Anti-human ABCB1 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| MHH-22-F(E) | Recombinant Human Anti-human ABCB1 Antibody Fab Fragment | WB, FC, FuncS | Fab |
| PFBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); Fab Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse IgG |
| PFBC-444 | Human Anti-ABCB1 Recombinant Antibody Fab Fragment (PFBC-444) | FC, Block, IP, IHC | Human Fab |
| HPAB-M0001-YC-F(E) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); Fab Fragment | IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-S(P) | Recombinant Anti-human ABCB1 Antibody scFv Fragment | Neut, IF, FuncS | scFv |
| MHH-22-S(P) | Recombinant Human Anti-human ABCB1 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| PSBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); scFv Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse scFv |
| PSBC-444 | Human Anti-ABCB1 Recombinant Antibody scFv Fragment (PSBC-444) | FC, Block, IP, IHC | Mouse scFv |
| HPAB-M0001-YC-S(P) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); scFv Fragment | IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-001CQ | Mouse Anti-ABCB1 Recombinant Antibody (clone UIC2) | FC, Block, IP, IHC | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0009 | Hi-Affi™ Rabbit Anti-ABCB1 Recombinant Antibody (clone DS9AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY6 | Anti-ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY16 | Anti-Mouse ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY15 | Anti-Human ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY17 | Anti-Goat ABCB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC423 | Recombinant Anti-ABCB1 Vesicular Antibody, EV Displayed (VS-0425-YC423) | Block, ELISA, FC, Cell-uptake |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, Inhib, FC
Application: ELISA, IHC, FC, IP, IF, Inhib
-2.png)
Application: WB, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
