Mouse Anti-ABCB1 Recombinant Antibody (HPAB-0076-YJ) (CAT#: HPAB-0076-YJ)
This product is a mouse antibody that recognizes a mapped epitope in the extracellular domain of human P-glycoprotein. The mAb is useful for the identification or purification of cells which express human Pgp, e.g. when contained in heterogeneous cell populations, and for monitoring the multi-drug resistant status of cells, e.g. tumor cells. This antibody can be used as a P-Glycoprotein inhibitor and functions in modulating cancer multidrug resistance.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
Figure 6 RNA cell line category: Cell line enhanced (Hep G2, RH-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
Specifications
- Immunogen
- Living intact human multidrug-resistant cells
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Human ABCB1
- Species Reactivity
- Human
- Applications
- IF, FC, IHC-P, ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody MM4.17 has been reported in applications of Immunofluorescence, Flow Cytometry, Immunohistochemistry-Paraffin, Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Flow Cytometry: MAb MM4.17 can be used at a concentration of 10 μg/mL.
Target
- Alternative Names
- CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; ATP-binding cassette, sub-family B (MDR/TAP), member 1; P glycoprotein; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance
- Gene ID
- 5243
- UniProt ID
- P08183
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ABCB1"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22 | Recombinant Anti-human ABCB1 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MOB-1496CT | Mouse Anti-ABCB1 Recombinant Antibody (clone 5F4) | FC, IF | Mouse IgG2a |
| MRO-1138-CN | Rabbit Anti-ABCB1 Recombinant Antibody (clone CBACN-413) | WB, IHC | Rabbit IgG |
| HPAB-M0001-YC | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6) | IHC | Mouse IgG |
| VS3-QX2 | Mouse Anti-ABCB1 Recombinant Antibody (clone 1G6) | WB, IP, IF | Mouse IgG1 |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-0076-YJ. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, FuncS
Application: WB, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, BL
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: IB, FC, ELISA, FuncS
Application: ELISA, Neut, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
