Mouse Anti-ABCG2 Recombinant Antibody (Clone 5D3)
CAT#: PABS-0024
This recombinant monoclonal antibody to human ABCG2 is a mouse monoclonal antibody that can be used for applications: ELISA.
Published Data
Gene Expression
IF
Figure 1 Alexa Fluor 647 conjugated ABCG2-specific antibody (5D3) was used to detect ABCG2 on the cell surface.
Scale bars represent 10 µm.
Bartos, Z., & Homolya, L. (2021). Identification of specific trafficking defects of naturally occurring variants of the human ABCG2 transporter. Frontiers in Cell and Developmental Biology, 9, 40.
Figure 2 Trafficking of the ABCG2 variants from the ER to the cell surface.
HeLa cells expressing the M71V-ABCG2 variants were labeled with various cellular markers in the absence (0 h) and after the addition of biotin (1-4 h). Representative confocal images show the cellular localization of the ABCG2 variant (green) and the marker (red). Scale bars represent 10 µm.
Bartos, Z., & Homolya, L. (2021). Identification of specific trafficking defects of naturally occurring variants of the human ABCG2 transporter. Frontiers in Cell and Developmental Biology, 9, 40.
Figure 3 Trafficking of the ABCG2 variants from the ER to the cell surface.
HeLa cells expressing the Q141K-ABCG2 variants were labeled with various cellular markers in the absence (0 h) and after the addition of biotin (1-4 h). Representative confocal images show the cellular localization of the ABCG2 variant (green) and the marker (red). Scale bars represent 10 µm.
Bartos, Z., & Homolya, L. (2021). Identification of specific trafficking defects of naturally occurring variants of the human ABCG2 transporter. Frontiers in Cell and Developmental Biology, 9, 40.
Figure 4 The cell surface labeling with 5D3 (G-I) as cellular markers.
Kin represent the time constants for entering the given compartment, respectively. Data represent mean ± SEM of three independent experiments involving 100-120 cells each. Asterisks indicate statistically significant differences (p < 0.05).
Bartos, Z., & Homolya, L. (2021). Identification of specific trafficking defects of naturally occurring variants of the human ABCG2 transporter. Frontiers in Cell and Developmental Biology, 9, 40.
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Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37299/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_B_7_5.jpg
Normal Tissue
Figure 4 Small intestine
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_4_2.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, MCF7, OE19, RPMI-8226, SuSa)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118777-ABCG2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ABCG2
- Species Reactivity
- Human
- Clone
- 5D3
- Applications
- ELISA, FuncS
- Related Disease
- Serum urate levels and risk of gout
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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- CAT
- Product Name
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| HPAB-M0002-YC-S(P) | Mouse Anti-ABCG2 Recombinant Antibody; scFv Fragment (HPAB-M0002-YC-S(P)) | ELISA, WB | Mouse scFv |
| PSBS-0024 | Mouse Anti-ABCG2 Recombinant Antibody (Clone 5D3); scFv Fragment | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
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| HPAB-M0002-YC-F(E) | Mouse Anti-ABCG2 Recombinant Antibody; Fab Fragment (HPAB-M0002-YC-F(E)) | ELISA, WB | Mouse Fab |
| PFBS-0024 | Mouse Anti-ABCG2 Recombinant Antibody (Clone 5D3); Fab Fragment | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
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