Mouse Anti-ABCG2 Recombinant Antibody (Clone 5D3) (CAT#: PABS-0024)
This recombinant monoclonal antibody to human ABCG2 is a mouse monoclonal antibody that can be used for applications: ELISA.
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IF
Figure 1 Alexa Fluor 647 conjugated ABCG2-specific antibody (5D3) was used to detect ABCG2 on the cell surface.
Scale bars represent 10 µm.
Bartos, Z., & Homolya, L. (2021). Identification of specific trafficking defects of naturally occurring variants of the human ABCG2 transporter. Frontiers in Cell and Developmental Biology, 9, 40.
Figure 2 Trafficking of the ABCG2 variants from the ER to the cell surface.
HeLa cells expressing the M71V-ABCG2 variants were labeled with various cellular markers in the absence (0 h) and after the addition of biotin (1-4 h). Representative confocal images show the cellular localization of the ABCG2 variant (green) and the marker (red). Scale bars represent 10 µm.
Bartos, Z., & Homolya, L. (2021). Identification of specific trafficking defects of naturally occurring variants of the human ABCG2 transporter. Frontiers in Cell and Developmental Biology, 9, 40.
Figure 3 Trafficking of the ABCG2 variants from the ER to the cell surface.
HeLa cells expressing the Q141K-ABCG2 variants were labeled with various cellular markers in the absence (0 h) and after the addition of biotin (1-4 h). Representative confocal images show the cellular localization of the ABCG2 variant (green) and the marker (red). Scale bars represent 10 µm.
Bartos, Z., & Homolya, L. (2021). Identification of specific trafficking defects of naturally occurring variants of the human ABCG2 transporter. Frontiers in Cell and Developmental Biology, 9, 40.
Figure 4 The cell surface labeling with 5D3 (G-I) as cellular markers.
Kin represent the time constants for entering the given compartment, respectively. Data represent mean ± SEM of three independent experiments involving 100-120 cells each. Asterisks indicate statistically significant differences (p < 0.05).
Bartos, Z., & Homolya, L. (2021). Identification of specific trafficking defects of naturally occurring variants of the human ABCG2 transporter. Frontiers in Cell and Developmental Biology, 9, 40.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ABCG2
- Species Reactivity
- Human
- Clone
- 5D3
- Applications
- ELISA, FuncS
- Related Disease
- Serum urate levels and risk of gout
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
- Gene ID
- 9429
- UniProt ID
- Q9UNQ0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| MOR-0016 | Hi-Affi™ Rabbit Anti-ABCG2 Recombinant Antibody (clone DS16AB) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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