Mouse Anti-ABL Recombinant Antibody (clone 2506W0209)
CAT#: VS-0625-FY314
The mouse anti-ABL monoclonal antibody is precisely developed for accurate detection in ELISA assays. It offers high specificity and sensitivity for the ABL (Abelson Murine Leukemia Viral Oncogene Homolog 1) protein, a non-receptor tyrosine kinase. This protein is a proto-oncogene involved in cell growth and differentiation. This antibody is valuable for researchers investigating cancer biology, signal transduction, and potential therapeutic targets. Its reliable binding ensures consistent and reproducible results, enabling detailed studies of cellular processes. This antibody is a useful tool for cancer research and cell biology investigations.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27280/259_B8_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28409/497_A9_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27251/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human prostate
Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27280/ihc_selected.jpg
Normal Tissue
Figure 5 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28409/ihc_selected.jpg
Normal Tissue
Figure 6 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2686/ihc_selected.jpg
Normal Tissue
Figure 7 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28409/61378_B_8_5.jpg
Normal Tissue
Figure 8 Adrenal gland
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28409/61378_B_5_5.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28409/61494_A_9_3.jpg
Normal Tissue
Figure 10 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28409/61494_A_9_4.jpg
Normal Tissue
Figure 11 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28409/61494_A_9_5.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000097007-ABL1
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Specifications
- Immunogen
- Abelson murine leukemia virus (A-MuLV) ABL
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- The antibody specifically recognizes the peptide of the Abelson murine leukemia virus (A-MuLV) ABL.
- Species Reactivity
- Abelson murine leukemia virus (A-MuLV)
- Clone
- 2506W0209
- Applications
- ELISA
Product Property
- Purification
- Purified with Protein A or G affinity chromatography
- Purity
- >90% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- The buffer commonly used is typically composed of 1 M Tris-HCl and 100 mM glycine. For more detailed information, refer to the Certificate of Analysis (COA).
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1:1000-1:3000
Other applications are under development. Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- Tyrosine-protein kinase transforming protein Abl; ABL
- UniProt ID
- P00521
- Long Name
- Tyrosine-protein kinase transforming protein Abl
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
See other products for "ABL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B048(A) | Recombinant Anti-human ABL Intrabody [(D-Arg)9] | FC, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B048(G) | Recombinant Anti-human ABL Intrabody [+36 GFP] | CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B048(T) | Recombinant Anti-human ABL Intrabody [Tat] | ELISA, IF, Neut, FuncS | scFv-Tat |
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