Mouse Anti-ACKR1 Recombinant Antibody (clone NaM185-2C3 (2C3)); scFv Fragment
CAT#: FAMAB-0008CQ-S(P)
Recombinant Mouse Antibody scFv Fragment clone NaM185-2C3 (2C3), which is specific to ACKR1. The minimal DARC epitope recognized by 2C3 MAb is linear and consists of five amino acids: FEDVW.
Gene Expression
Normal Tissue
Figure 1 High expression in smooth muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16421/36523_B_4_7_val_selected.jpg
Normal Tissue
Figure 2 Low expression in kidney
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16421/36523_A_8_5_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16421/36523_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Proximal tubules (microvilli) Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16421/36523_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16421/36523_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HEL, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213088-ACKR1
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Specifications
- Immunogen
- CHO cells expressing the recombinant Duffy glycoprotein
- Host Species
- Mouse
- Type
- Mouse scFv
- Specificity
- Human ACKR1
- Species Reactivity
- Human
- Clone
- NaM185-2C3 (2C3)
- Applications
- ELISA, FC, Block
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Atypical Chemokine Receptor 1 (Duffy Blood Group); Duffy Blood Group, Atypical Chemokine Receptor; Duffy Blood Group, Chemokine Receptor; Plasmodium Vivax Receptor; Fy Glycoprotein; Glycoprotein D; DARC; GpFy; GPD; FY; Duffy Blood Group Chemokine Receptor; Duffy Antigen/Chemokine Receptor; Atypical Chemokine Receptor 1; Duffy Blood Group Antigen; Duffy Blood Group; CD234 Antigen; CCBP1; CD234; WBCQ1; Dfy
- Gene ID
- 2532
- UniProt ID
- Q16570
REVIEWS AND Q&AS
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Customer Review
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No Non-Specific Bands
We appreciated that there were no non-specific bands in our Western blots, which significantly improved our data quality.
Easy Protocol
Following the protocol provided with the antibody was straightforward, and the results were as expected. Highly recommended.
Robust Data
The data obtained using this antibody were robust, reproducible, and reliable, which is critical for our research.
Q&As
-
How do I store reconstituted antibody solutions?
A: Store reconstituted solutions in small aliquots at -20°C or lower to avoid freeze-thaw cycles. Ensure solutions are tightly sealed to prevent contamination and degradation.
-
What are the advantages of using recombinant antibodies?
A: Recombinant antibodies offer high specificity, batch consistency, and reduced risk of contamination, making them reliable for various research applications.
-
What steps should I take if the antibody isn't producing expected results?
A: Recheck all steps, including sample preparation, antibody concentration, incubation times, and detection methods. Troubleshooting often involves optimizing each of these parameters.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone NaM185-2C3 (2C3)"
- CAT
- Product Name
See other products for "ACKR1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1354z | Mouse Anti-ACKR1 Recombinant Antibody (clone 9C5) | WB, ELISA, FC, ICC, IF | Mouse IgG1, κ |
| FAMAB-0007CQ | Human Anti-ACKR1 Recombinant Antibody (clone ch2C3) | ELISA, FC, Block | Chimeric (mouse/human) IgG4, κ |
| FAMAB-0008CQ | Mouse Anti-ACKR1 Recombinant Antibody (clone NaM185-2C3 (2C3)) | ELISA, WB, FC, Block | Mouse IgG1, κ |
| FAMAB-1017CQ | Human Anti-ACKR1 Recombinant Antibody (clone CA52) | Block, SPR, WB, ELISA | Human IgG1 |
| FAMAB-1352CQ | Mouse Anti-ACKR1 Recombinant Antibody (clone Hima-19) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-008 | Recombinant Anti-Human ACKR1 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0927 | Hi-Affi™ Rabbit Anti-ACKR1 Recombinant Antibody (clone DS927AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0007CQ-F(E) | Human Anti-ACKR1 Recombinant Antibody (clone ch2C3); Fab Fragment | ELISA, FC, Block | Chimeric (mouse/human) Fab |
| FAMAB-0008CQ-F(E) | Mouse Anti-ACKR1 Recombinant Antibody (clone NaM185-2C3 (2C3)); Fab Fragment | ELISA, FC, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC16 | CytoStream™ Rabbit Anti-ACKR1 Recombinant Antibody (VS13-YC16) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC734 | Recombinant Anti-ACKR1 Vesicular Antibody, EV Displayed (VS-0425-YC734) | Block, ELISA, FC, SPR, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY76 | Anti-ACKR1 Immunohistochemistry Kit | IHC |
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