Rabbit Anti-ACLY (pT447/pS451) Recombinant Antibody (clone R01-1B5)
CAT#: VS3-WK1994
This product is a rabbit recombinant antibody that is specific to phosphorylated ACLY. The target of this antibody is phospho-ACLY (Thr447/Ser451). The clone R01-1B5 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of ACLY (pThr447/pSer451) observed in WB experiments using this antibody is 121 kDa.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28758/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7783/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/395_D3_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/399_D3_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_9_3.jpg
Normal Tissue
Figure 6 Pancreas
Pancreatic endocrine cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131473-ACLY
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Thr447/Ser451 of human ATP citrate lyase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr447/Ser451 of human ACLY
- Species Reactivity
- Human
- Clone
- R01-1B5
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "ACLY"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-17-VHH | Recombinant Anti-human ACLY VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0038 | Hi-Affi™ Rabbit Anti-ACLY Recombinant Antibody (clone DS38AB) | WB, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1666-CN | Rabbit Anti-ACLY Polyclonal Antibody (MRO-1666-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0526J | Rabbit Anti-ACLY Recombinant Antibody (clone 3A5) | ELISA, WB, IF, FC | Rabbit IgG |
| VS3-QX6 | Mouse Anti-ACLY Recombinant Antibody (clone 5F8D11) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ1034 | Rabbit Anti-ACLY Recombinant Antibody (VS3-CJ1034) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS7-HM16 | Mouse Anti-ACLY Recombinant Antibody (clone CBL010HM) | WB, IF, ICC, FCM | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC691 | AbPlus™ Anti-ACLY Magnetic Beads (VS-0724-YC691) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY21 | Anti-ACLY Immunohistochemistry Kit | IHC | |
| VS-0525-XY78 | Anti-Mouse ACLY Immunohistochemistry Kit | IHC | |
| VS-0525-XY77 | Anti-Human ACLY Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC17 | CytoStream™ Mouse Anti-ACLY Recombinant Antibody (VS13-YC17) | WB, IF, FC | Mouse IgG2a |
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